Development of hydroxyapatite-coated nonwovens for efficient isolation of somatic stem cells from adipose tissues

Ryota Chijimatsu, Taiga Takeda, Shinsaku Tsuji, Kohei Sasaki, Koichi Kato, Rie Kojima, Noriko Michihata, Toshiya Tsubaki, Aya Matui, Miharu Watanabe, Sakae Tanaka, Taku Saito

Research output: Contribution to journalArticlepeer-review


Adipose-derived stem cells (ASCs) are an attractive cell source for cell therapy. Despite the increasing number of clinical applications, the methodology for ASC isolation is not optimized for every individual. In this study, we developed an effective material to stabilize explant cultures from small-fragment adipose tissues. Methods: Polypropylene/polyethylene nonwoven sheets were coated with hydroxyapatite (HA) particles. Adipose fragments were then placed on these sheets, and their ability to trap tissue was monitored during explant culture. The yield and properties of the cells were compared to those of cells isolated by conventional collagenase digestion. Results: Hydroxyapatite-coated nonwovens immediately trapped adipose fragments when placed on the sheets. The adhesion was stable even in culture media, leading to cell migration and proliferation from the tissue along with the nonwoven fibers. A higher fiber density further enhanced cell growth. Although cells on nonwoven explants could not be fully collected with cell dissociation enzymes, the cell yield was significantly higher than that of conventional monolayer culture without impacting stem cell properties. Conclusions: Hydroxyapatite-coated nonwovens are useful for the effective primary explant culture of connective tissues without enzymatic cell dissociation.

Original languageEnglish
Pages (from-to)52-61
Number of pages10
JournalRegenerative Therapy
Publication statusPublished - Dec 2022
Externally publishedYes


  • Adipose stem cells
  • Explant culture
  • Hydroxyapatite
  • Nonwovens

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Developmental Biology


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