Dietary isothiocyanates modify mitochondrial functions through their electrophilic reaction

Makiko Kawakami; Nobuhiro Harada; Mio Hiratsuka; Kiyoshi Kawai; Yoshimasa Nakamura

doi:10.1271/bbb.69.2439

Dietary isothiocyanates modify mitochondrial functions through their electrophilic reaction

Makiko Kawakami, Nobuhiro Harada, Mio Hiratsuka, Kiyoshi Kawai, Yoshimasa Nakamura

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

We found that both benzyl isothiocyanate (ITC) and phenyl ITC inhibited respiration in the mitochondria in an electrophilic reaction-dependent manner. ITC-induced mitochondrial swelling and cytochrome c release were prevented by cyclosporin A, indicating that they are mediated through the ITC moiety-dependent reaction to critical thiol groups for the opening of membrane permeability transition-dependent pores.

Original language	English
Pages (from-to)	2439-2444
Number of pages	6
Journal	Bioscience, Biotechnology and Biochemistry
Volume	69
Issue number	12
DOIs	https://doi.org/10.1271/bbb.69.2439
Publication status	Published - 2005

Keywords

Cytochrome c
Electrophile
Isothiocyanate
Membrane permeability transition
Mitochondria

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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title = "Dietary isothiocyanates modify mitochondrial functions through their electrophilic reaction",

abstract = "We found that both benzyl isothiocyanate (ITC) and phenyl ITC inhibited respiration in the mitochondria in an electrophilic reaction-dependent manner. ITC-induced mitochondrial swelling and cytochrome c release were prevented by cyclosporin A, indicating that they are mediated through the ITC moiety-dependent reaction to critical thiol groups for the opening of membrane permeability transition-dependent pores.",

keywords = "Cytochrome c, Electrophile, Isothiocyanate, Membrane permeability transition, Mitochondria",

author = "Makiko Kawakami and Nobuhiro Harada and Mio Hiratsuka and Kiyoshi Kawai and Yoshimasa Nakamura",

note = "Funding Information: This study was supported by a grant-in-aid for Encouragement of Young Scientists (A) (no. 17688006) from the Ministry of Education, Culture, Sports, Science and Technology of the Japanese Government.",

year = "2005",

doi = "10.1271/bbb.69.2439",

language = "English",

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T1 - Dietary isothiocyanates modify mitochondrial functions through their electrophilic reaction

AU - Kawakami, Makiko

AU - Harada, Nobuhiro

AU - Hiratsuka, Mio

AU - Kawai, Kiyoshi

AU - Nakamura, Yoshimasa

N1 - Funding Information: This study was supported by a grant-in-aid for Encouragement of Young Scientists (A) (no. 17688006) from the Ministry of Education, Culture, Sports, Science and Technology of the Japanese Government.

PY - 2005

Y1 - 2005

N2 - We found that both benzyl isothiocyanate (ITC) and phenyl ITC inhibited respiration in the mitochondria in an electrophilic reaction-dependent manner. ITC-induced mitochondrial swelling and cytochrome c release were prevented by cyclosporin A, indicating that they are mediated through the ITC moiety-dependent reaction to critical thiol groups for the opening of membrane permeability transition-dependent pores.

AB - We found that both benzyl isothiocyanate (ITC) and phenyl ITC inhibited respiration in the mitochondria in an electrophilic reaction-dependent manner. ITC-induced mitochondrial swelling and cytochrome c release were prevented by cyclosporin A, indicating that they are mediated through the ITC moiety-dependent reaction to critical thiol groups for the opening of membrane permeability transition-dependent pores.

KW - Cytochrome c

KW - Electrophile

KW - Isothiocyanate

KW - Membrane permeability transition

KW - Mitochondria

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AN - SCOPUS:29344443507

SN - 0916-8451

VL - 69

SP - 2439

EP - 2444

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 12

ER -

Dietary isothiocyanates modify mitochondrial functions through their electrophilic reaction

Abstract

Keywords

ASJC Scopus subject areas

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