TY - JOUR
T1 - Differential effects of flagellins from Pseudomonas syringae pv. tabaci, tomato and glycinea on plant defense response
AU - Taguchi, Fumiko
AU - Shimizu, Rena
AU - Nakajima, Rie
AU - Toyoda, Kazuhiro
AU - Shiraishi, Tomonori
AU - Ichinose, Yuki
N1 - Funding Information:
We thank Dr. J.D. Taylor (Institute of Horticultural Research, UK), Dr. A. Collmer (Cornell University, USA) and Japan Tobacco Inc., Leaf Tobacco Research Laboratory for providing P . syringae pv. pisi, pv. glycinea and tomato , and pv. tabaci, respectively. We thank Drs. K. Akimitsu (Kagawa University, Japan) and T. Kunieda (Okayama University, Japan) for the generation of anti-flagellin antibody and Drs. F.-S. Che (Nara Institute of Science and Technology, Japan), T. Mukaihara (RIBS, Okayama), S. Aizawa (Teikyo University, Japan) and Y. Inagaki (Okayama University, Okayama) for valuable comments. We are also grateful to Dr. M. Sasabe for technical assistance. This work was supported in part by a Grant-in-Aid for Scientific Research on Priority Areas (no. 12052215) from the Ministry of Education, Science and Culture, Japan and the Japan Society for the Promotion of Science.
PY - 2003/2/1
Y1 - 2003/2/1
N2 - To investigate the factor that determines incompatible interactions between Pseudomonas syringae pv. tabaci and non-host plants, an elicitor of hypersensitive reaction (HR) was partially purified from the supernatant of a nutrient-poor medium of bacterial culture by DEAE column chromatography. The major protein in the elicitor-active fractions was identified as a flagellin which is a component of flagellar filaments. The flagellins purified from P. syringae pv. tomato and glycinea, incompatible pathogens of tobacco plants, induced fragmentation of chromosomal DNA and oxidative burst accompanied by programmed cell death in tobacco (Nicotiana tabacum) Bright Yellow (BY-2) cells, but the flagellin from pv. tabaci, a compatible pathogen, did not. However, the amino acid sequences of flagellins deduced from fliC genes showed a high homology among these P. syringae pathovars. In particular, the amino acid sequences of pv. tabaci and glycinea are completely identical. However, both recombinant flagellins produced in Escherichia coli possess HR-inducing activity in BY-2 cells. These results indicate that the post-translational modification of flagellins has an important role for HR-inducing ability in tobacco cells. Furthermore, we discuss the cause of a different elicitor activity among flagellins on tobacco cells and the role of flagellins in the determining specificity.
AB - To investigate the factor that determines incompatible interactions between Pseudomonas syringae pv. tabaci and non-host plants, an elicitor of hypersensitive reaction (HR) was partially purified from the supernatant of a nutrient-poor medium of bacterial culture by DEAE column chromatography. The major protein in the elicitor-active fractions was identified as a flagellin which is a component of flagellar filaments. The flagellins purified from P. syringae pv. tomato and glycinea, incompatible pathogens of tobacco plants, induced fragmentation of chromosomal DNA and oxidative burst accompanied by programmed cell death in tobacco (Nicotiana tabacum) Bright Yellow (BY-2) cells, but the flagellin from pv. tabaci, a compatible pathogen, did not. However, the amino acid sequences of flagellins deduced from fliC genes showed a high homology among these P. syringae pathovars. In particular, the amino acid sequences of pv. tabaci and glycinea are completely identical. However, both recombinant flagellins produced in Escherichia coli possess HR-inducing activity in BY-2 cells. These results indicate that the post-translational modification of flagellins has an important role for HR-inducing ability in tobacco cells. Furthermore, we discuss the cause of a different elicitor activity among flagellins on tobacco cells and the role of flagellins in the determining specificity.
KW - Cell death
KW - Elicitor
KW - Flagellin
KW - FliC
KW - Hypersensitive reaction
KW - Pseudomonas syringae
KW - Tobacco
UR - http://www.scopus.com/inward/record.url?scp=0037303481&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037303481&partnerID=8YFLogxK
U2 - 10.1016/S0981-9428(02)00018-9
DO - 10.1016/S0981-9428(02)00018-9
M3 - Article
AN - SCOPUS:0037303481
SN - 0981-9428
VL - 41
SP - 165
EP - 174
JO - Plant Physiology and Biochemistry
JF - Plant Physiology and Biochemistry
IS - 2
ER -