TY - JOUR
T1 - Differential effects of orally administered Lactobacillus acidophilus L-55 on the gene expression of cytokines and master immune switches in the ileum and spleen of laying hen with an attenuated Newcastle disease virus vaccine
AU - Pham, Hung Hoang Son
AU - Fujii, Yusuke
AU - Arakawa, Kensuke
AU - Hatabu, Toshimitsu
N1 - Funding Information:
We would like to thank Dr. Dung Thi Ho at Hue University of Agriculture and Forestry and Ms. Nodoka Koshio at theLaboratory of Animal Physiology of Okayama University fortheir technical help. The authors would like to thank Enago(www.enago.jp) for the English language review
Publisher Copyright:
© 2022 BMFH Press
PY - 2022
Y1 - 2022
N2 - This study aimed to evaluate the benefits of oral administration of Lactobacillus acidophilus strain L-55 (LaL-55) to chickens inoculated with a Newcastle disease virus (NDV)-based live-attenuated vaccine by examiningthe mRNA expression of several genes related to viral infection in the spleen and ileum by quantitative reversetranscription polymerase chain reaction. In the spleen, interferon (IFN)-α was significantly higher in the low and middle-dose LaL-55 groups at 6 weeks than at 4 weeks. IFN regulatory factor (IRF)-3 and IRF-7 expressionwas significantly higher in the low-dose LaL-55 group than in the middle and high-dose LaL-55 groups. In theileum, melanoma differentiation-associated gene 5 showed a dose-dependent increase at 4 weeks. IFN-γ and IRF-7showed dose-dependent increases at 6 weeks. These results suggested that LaL-55 boosts the immune response tothe NDV vaccine, albeit by different mechanisms in the spleen and ileum
AB - This study aimed to evaluate the benefits of oral administration of Lactobacillus acidophilus strain L-55 (LaL-55) to chickens inoculated with a Newcastle disease virus (NDV)-based live-attenuated vaccine by examiningthe mRNA expression of several genes related to viral infection in the spleen and ileum by quantitative reversetranscription polymerase chain reaction. In the spleen, interferon (IFN)-α was significantly higher in the low and middle-dose LaL-55 groups at 6 weeks than at 4 weeks. IFN regulatory factor (IRF)-3 and IRF-7 expressionwas significantly higher in the low-dose LaL-55 group than in the middle and high-dose LaL-55 groups. In theileum, melanoma differentiation-associated gene 5 showed a dose-dependent increase at 4 weeks. IFN-γ and IRF-7showed dose-dependent increases at 6 weeks. These results suggested that LaL-55 boosts the immune response tothe NDV vaccine, albeit by different mechanisms in the spleen and ileum
KW - Ileum
KW - Irf
KW - Lactobacillus acidophilus
KW - Mda5
KW - Newcastle disease virus
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U2 - 10.12938/BMFH.2021-026
DO - 10.12938/BMFH.2021-026
M3 - Article
AN - SCOPUS:85123517755
SN - 2186-6953
VL - 41
SP - 12
EP - 19
JO - Bioscience of Microbiota, Food and Health
JF - Bioscience of Microbiota, Food and Health
IS - 1
ER -
