TY - JOUR
T1 - Differential Expression of S100C in Thyroid Lesions
AU - Torres-Cabala, Carlos
AU - Panizo-Santos, Angel
AU - Krutzsch, Henry C.
AU - Barazi, Heba
AU - Namba, Masayoshi
AU - Sakaguchi, Masakiyo
AU - Roberts, David D.
AU - Merino, Maria J.
PY - 2004/4
Y1 - 2004/4
N2 - Identification of new potential markers that may help in the diagnosis of benign and malignant thyroid lesions is needed. By comparative 2-dimensional gel electrophoresis of microdissected cells from tumors and normal thyroid tissue, we identified a new protein, S100C, which is highly expressed in papillary carcinomas. In order to validate this finding, we investigated the immunohistochemical expression and the potential role in diagnosis of these markers in 94 specimens representing the spectrum of malignant and benign thyroid lesions. Normal thyroid tissue was evaluated in 57 specimens. Galectin-3, a marker reported as specific for malignant lesions, was also evaluated in the same lesions. S100C protein was expressed in the nuclei of normal tissue, hyperplastic nodules, and follicular adenomas and carcinomas. Papillary carcinomas showed a strong, but cytoplasmic, pattern of staining. Galectin-3 immunostaining was strongly positive in papillary carcinomas, and negative in benign lesions, confirming its value in differential diagnosis. These findings suggest that immunohistochemical staining of S100C could be helpful in the pathological study of thyroid lesions, especially in cases in which follicular variants of papillary carcinoma and follicular carcinoma are considered in the differential diagnosis.
AB - Identification of new potential markers that may help in the diagnosis of benign and malignant thyroid lesions is needed. By comparative 2-dimensional gel electrophoresis of microdissected cells from tumors and normal thyroid tissue, we identified a new protein, S100C, which is highly expressed in papillary carcinomas. In order to validate this finding, we investigated the immunohistochemical expression and the potential role in diagnosis of these markers in 94 specimens representing the spectrum of malignant and benign thyroid lesions. Normal thyroid tissue was evaluated in 57 specimens. Galectin-3, a marker reported as specific for malignant lesions, was also evaluated in the same lesions. S100C protein was expressed in the nuclei of normal tissue, hyperplastic nodules, and follicular adenomas and carcinomas. Papillary carcinomas showed a strong, but cytoplasmic, pattern of staining. Galectin-3 immunostaining was strongly positive in papillary carcinomas, and negative in benign lesions, confirming its value in differential diagnosis. These findings suggest that immunohistochemical staining of S100C could be helpful in the pathological study of thyroid lesions, especially in cases in which follicular variants of papillary carcinoma and follicular carcinoma are considered in the differential diagnosis.
KW - Calgizzarin
KW - Galectin-3
KW - Immunohistochemistry
KW - Proteomics
KW - S100C
KW - Thyroid
UR - http://www.scopus.com/inward/record.url?scp=2142717373&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2142717373&partnerID=8YFLogxK
U2 - 10.1177/106689690401200203
DO - 10.1177/106689690401200203
M3 - Article
C2 - 15173915
AN - SCOPUS:2142717373
SN - 1066-8969
VL - 12
SP - 107
EP - 115
JO - International Journal of Surgical Pathology
JF - International Journal of Surgical Pathology
IS - 2
ER -