TY - JOUR
T1 - Differential expression of the two closely related LIM-Class homeobox genes LH-2A and LH-2B during limb development
AU - Nohno, Tsutomu
AU - Kawakami, Yasuhiko
AU - Wada, Naoyuki
AU - Ishikawa, Tetsuya
AU - Ohuchi, Hideyo
AU - Noji, Sumihare
N1 - Funding Information:
We thank Rie Nakao and Chikako Komaguchi for their technical assistance. This work was supported in part by Grants-in-Aids from the Japanese Ministry of Education, Science and Culture, and by a Research Project Grant from Kawasaki Medical School.
PY - 1997/9/18
Y1 - 1997/9/18
N2 - We have isolated the chicken homeobox genes LH-2A and LH-2B encoding two related LIM domain-containing homeodomain proteins and examined the expression pattern during chick limb development. LH-2A is most closely related to human and rat LH-2, while LH-2B is less conserved. Although both LH-2A and LH-2B are expressed in the limb mesenchyme throughout stage 16 to stage 32, LH-2A transcripts are detectable in the distal limb bud and LH-2B transcripts are detectable in the anterior limb bud. Signals from the apical ectodermal ridge positively regulate LH-2A expression, since removal of the apical ectoderm resulted in the rapid reduction of LH-2A expression in the distal limb mesenchyme. Ectopic expression of the sonic hedgehog gene in the anterior margin of the limb bud resulted in the rapid reduction of LH-2B expression accompanying respecification of the positional value to the posterior phenotype. These results suggest that LH-2A and LH-2B play important roles in the determination and specification of the proximal-distal and anterior-posterior positional values, respectively.
AB - We have isolated the chicken homeobox genes LH-2A and LH-2B encoding two related LIM domain-containing homeodomain proteins and examined the expression pattern during chick limb development. LH-2A is most closely related to human and rat LH-2, while LH-2B is less conserved. Although both LH-2A and LH-2B are expressed in the limb mesenchyme throughout stage 16 to stage 32, LH-2A transcripts are detectable in the distal limb bud and LH-2B transcripts are detectable in the anterior limb bud. Signals from the apical ectodermal ridge positively regulate LH-2A expression, since removal of the apical ectoderm resulted in the rapid reduction of LH-2A expression in the distal limb mesenchyme. Ectopic expression of the sonic hedgehog gene in the anterior margin of the limb bud resulted in the rapid reduction of LH-2B expression accompanying respecification of the positional value to the posterior phenotype. These results suggest that LH-2A and LH-2B play important roles in the determination and specification of the proximal-distal and anterior-posterior positional values, respectively.
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U2 - 10.1006/bbrc.1997.7320
DO - 10.1006/bbrc.1997.7320
M3 - Article
C2 - 9299541
AN - SCOPUS:0031577538
SN - 0006-291X
VL - 238
SP - 506
EP - 511
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -