Direct Detection of Epstein‐Barr Virus DNA from a Single Reed‐Sternberg Cell of Hodgkin's Disease by Polymerase Chain Reaction

Norihiro Teramoto; Tadaatsu Akagi; Tadashi Yoshino; Kiyoshi Takahashi; Ho Jong Jeon

doi:10.1111/j.1349-7006.1992.tb00110.x

Direct Detection of Epstein‐Barr Virus DNA from a Single Reed‐Sternberg Cell of Hodgkin's Disease by Polymerase Chain Reaction

Norihiro Teramoto, Tadaatsu Akagi, Tadashi Yoshino, Kiyoshi Takahashi, Ho Jong Jeon

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Eleven cases of Hodgkin's disease (HD) were examined for the presence of the Epstein‐Barr virus (EBV) genome, using the polymerase chain reaction (PCR) to detect EBV DNA in whole paraffin‐embedded tissue specimens and in single cells picked out from the specimens with a micromanipulator. The EBV genome was detected in 5 of the 11 cases by conventional PCR. Single cell PCR demonstrated the EBV genome in Reed‐Sternberg cells from all the EBV‐positive cases, but not from any of the EBV‐negative cases. Background lymphocytes and lysozyme‐positive histiocytes from EBV‐positive cases did not contain the EBV genome. These results indicate an etiological association of EBV with some cases of HD.

Original language	English
Pages (from-to)	329-333
Number of pages	5
Journal	Japanese Journal of Cancer Research
Volume	83
Issue number	4
DOIs	https://doi.org/10.1111/j.1349-7006.1992.tb00110.x
Publication status	Published - Apr 1992

Keywords

Hodgkin's disease
Key words
Micromanipulator
PCR ‐ Epstein‐Barr virus

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/j.1349-7006.1992.tb00110.x

Cite this

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abstract = "Eleven cases of Hodgkin's disease (HD) were examined for the presence of the Epstein‐Barr virus (EBV) genome, using the polymerase chain reaction (PCR) to detect EBV DNA in whole paraffin‐embedded tissue specimens and in single cells picked out from the specimens with a micromanipulator. The EBV genome was detected in 5 of the 11 cases by conventional PCR. Single cell PCR demonstrated the EBV genome in Reed‐Sternberg cells from all the EBV‐positive cases, but not from any of the EBV‐negative cases. Background lymphocytes and lysozyme‐positive histiocytes from EBV‐positive cases did not contain the EBV genome. These results indicate an etiological association of EBV with some cases of HD.",

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author = "Norihiro Teramoto and Tadaatsu Akagi and Tadashi Yoshino and Kiyoshi Takahashi and Jeon, {Ho Jong}",

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AU - Yoshino, Tadashi

AU - Takahashi, Kiyoshi

AU - Jeon, Ho Jong

PY - 1992/4

Y1 - 1992/4

N2 - Eleven cases of Hodgkin's disease (HD) were examined for the presence of the Epstein‐Barr virus (EBV) genome, using the polymerase chain reaction (PCR) to detect EBV DNA in whole paraffin‐embedded tissue specimens and in single cells picked out from the specimens with a micromanipulator. The EBV genome was detected in 5 of the 11 cases by conventional PCR. Single cell PCR demonstrated the EBV genome in Reed‐Sternberg cells from all the EBV‐positive cases, but not from any of the EBV‐negative cases. Background lymphocytes and lysozyme‐positive histiocytes from EBV‐positive cases did not contain the EBV genome. These results indicate an etiological association of EBV with some cases of HD.

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KW - Key words

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Direct Detection of Epstein‐Barr Virus DNA from a Single Reed‐Sternberg Cell of Hodgkin's Disease by Polymerase Chain Reaction

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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