Direct evidence of cytosolic PNGase activity in Arabidopsis thaliana: In vitro assay system for plant cPNGase activity

Sahoko Shirai; Ryota Uemura; Megumi Maeda; Hiroyuki Kajiura; Ryo Misaki; Kazuhito Fujiyama; Yoshinobu Kimura

doi:10.1093/bbb/zbab047

Direct evidence of cytosolic PNGase activity in Arabidopsis thaliana: In vitro assay system for plant cPNGase activity

Sahoko Shirai, Ryota Uemura, Megumi Maeda, Hiroyuki Kajiura, Ryo Misaki, Kazuhito Fujiyama, Yoshinobu Kimura

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Cytosolic peptide:N-glycanase (cPNGase), which occurs ubiquitously in eukaryotic cells, is involved in the de-N-glycosylation of misfolded glycoproteins in the protein quality control system. In this study, we aimed to provide direct evidence of plant cPNGase activity against a denatured glycoprotein using a crude extract prepared from a mutant line of Arabidopsis thaliana lacking 2 acidic PNGase genes.

Original language	English
Pages (from-to)	1460-1463
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	85
Issue number	6
DOIs	https://doi.org/10.1093/bbb/zbab047
Publication status	Published - Jun 1 2021

Keywords

Arabidopsis thaliana
PNGase assay
acidic PNGase
cytosolic PNGase
free N-glycan

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1093/bbb/zbab047

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abstract = "Cytosolic peptide:N-glycanase (cPNGase), which occurs ubiquitously in eukaryotic cells, is involved in the de-N-glycosylation of misfolded glycoproteins in the protein quality control system. In this study, we aimed to provide direct evidence of plant cPNGase activity against a denatured glycoprotein using a crude extract prepared from a mutant line of Arabidopsis thaliana lacking 2 acidic PNGase genes. ",

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AU - Shirai, Sahoko

AU - Uemura, Ryota

AU - Maeda, Megumi

AU - Kajiura, Hiroyuki

AU - Misaki, Ryo

AU - Fujiyama, Kazuhito

AU - Kimura, Yoshinobu

PY - 2021/6/1

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AB - Cytosolic peptide:N-glycanase (cPNGase), which occurs ubiquitously in eukaryotic cells, is involved in the de-N-glycosylation of misfolded glycoproteins in the protein quality control system. In this study, we aimed to provide direct evidence of plant cPNGase activity against a denatured glycoprotein using a crude extract prepared from a mutant line of Arabidopsis thaliana lacking 2 acidic PNGase genes.

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KW - acidic PNGase

KW - cytosolic PNGase

KW - free N-glycan

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Direct evidence of cytosolic PNGase activity in Arabidopsis thaliana: In vitro assay system for plant cPNGase activity

Abstract

Keywords

ASJC Scopus subject areas

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