TY - JOUR
T1 - Direct interaction analysis of microRNA-351-5p and nuclear scaffold lamin B1 mRNA by the cell-free in vitro mRNA/miRNA binding evaluation system
AU - Sato, Akira
AU - Ogino, Yoko
AU - Shimotsuma, Akira
AU - Hiramoto, Akiko
AU - Kim, Hye Sook
AU - Wataya, Yusuke
N1 - Funding Information:
We thank Dr. Hikoya Hayatsu (Okayama University) for helpful discussions. This research was funded by JSPS KAKENHI, grant number JP21790078 (1stAS), JP24790216 (1stAS), JP26460310 (1stAS) and JP17K08550 (1stAS). This work was also supported by a Research Education Fund (1stAS) and Experimental Research Fund (1stAS) for Tokyo University of Science. We would also like to thank Enago (www.enago.jp) for English language editing.
Funding Information:
We thank Dr. Hikoya Hayatsu (Okayama University) for helpful discussions. This research was funded by JSPS KAKENHI, grant number JP21790078 (1AS), JP24790216 (1AS), JP26460310 (1AS) and JP17K08550 (1AS). This work was also supported by a Research Education Fund (1AS) and Experimental Research Fund (1AS) for Tokyo University of Science. We would also like to thank Enago ( www.enago.jp ) for English language editing. st st st st st st
Publisher Copyright:
© 2019, © 2019 Taylor & Francis Group, LLC.
PY - 2020/6/2
Y1 - 2020/6/2
N2 - We previously demonstrated that miR-351-5p regulates nuclear scaffold lamin B1 expression and mediates the anticancer floxuridine-induced necrosis shift to apoptosis in mammalian tumor cells. Notably, it is unknown whether lamin B1 mRNA is a direct target of miR-351-5p. Here, we show that miR-351-5p interacts with a lamin B1 mRNA partial sequence by using the cell-free in vitro miRNA and mRNA binding evaluation system. In addition, the interaction of miR-351-5p/lamin B1 mRNA was suppressed by an miR-351-5p inhibitor. Our findings are important in exploring the functions of miRNAs in cellular processes, including cell death.
AB - We previously demonstrated that miR-351-5p regulates nuclear scaffold lamin B1 expression and mediates the anticancer floxuridine-induced necrosis shift to apoptosis in mammalian tumor cells. Notably, it is unknown whether lamin B1 mRNA is a direct target of miR-351-5p. Here, we show that miR-351-5p interacts with a lamin B1 mRNA partial sequence by using the cell-free in vitro miRNA and mRNA binding evaluation system. In addition, the interaction of miR-351-5p/lamin B1 mRNA was suppressed by an miR-351-5p inhibitor. Our findings are important in exploring the functions of miRNAs in cellular processes, including cell death.
KW - MicroRNA/mRNA interaction
KW - lamin B1 mRNA
KW - miRNA inhibitor
KW - miRNA-351-5p
UR - http://www.scopus.com/inward/record.url?scp=85078408390&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85078408390&partnerID=8YFLogxK
U2 - 10.1080/15257770.2019.1702675
DO - 10.1080/15257770.2019.1702675
M3 - Article
C2 - 31994437
AN - SCOPUS:85078408390
SN - 1525-7770
VL - 39
SP - 799
EP - 805
JO - Nucleosides, Nucleotides and Nucleic Acids
JF - Nucleosides, Nucleotides and Nucleic Acids
IS - 6
ER -