Direct targeting of proteins to lipid droplets demonstrated by time-lapse live cell imaging

Torahiko Tanaka; Kazumichi Kuroda; Masanori Ikeda; Nobuyuki Kato; Kazufumi Shimizu; Makoto Makishima

doi:10.1016/j.jbiosc.2013.05.006

Direct targeting of proteins to lipid droplets demonstrated by time-lapse live cell imaging

Torahiko Tanaka, Kazumichi Kuroda, Masanori Ikeda, Nobuyuki Kato, Kazufumi Shimizu, Makoto Makishima

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

A protein that specifically targets lipid droplets (LDs) was created by connecting two domains of nonstructural protein 4B containing amphipathic helices from hepatitis C virus. We demonstrated its direct targeting and accumulation to the LD surface by time-lapse live cell imaging, comparable to those observed with adipose differentiation-related protein.

Original language	English
Pages (from-to)	620-623
Number of pages	4
Journal	Journal of Bioscience and Bioengineering
Volume	116
Issue number	5
DOIs	https://doi.org/10.1016/j.jbiosc.2013.05.006
Publication status	Published - Nov 2013
Externally published	Yes

Keywords

Adipose differentiation-related protein
Hepatitis C virus
Lipid droplets
Nonstructural protein 4B
Time-lapse imaging

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jbiosc.2013.05.006

Cite this

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title = "Direct targeting of proteins to lipid droplets demonstrated by time-lapse live cell imaging",

abstract = "A protein that specifically targets lipid droplets (LDs) was created by connecting two domains of nonstructural protein 4B containing amphipathic helices from hepatitis C virus. We demonstrated its direct targeting and accumulation to the LD surface by time-lapse live cell imaging, comparable to those observed with adipose differentiation-related protein.",

keywords = "Adipose differentiation-related protein, Hepatitis C virus, Lipid droplets, Nonstructural protein 4B, Time-lapse imaging",

author = "Torahiko Tanaka and Kazumichi Kuroda and Masanori Ikeda and Nobuyuki Kato and Kazufumi Shimizu and Makoto Makishima",

note = "Funding Information: Technical assistance by K. Toyosawa is gratefully acknowledged. We thank Drs. M. Moriyama and T. Hayashida for valuable comments. This work was supported by Strategic Research Base Development Program for Private Universities subsidized by the Ministry of Education, Culture, Sports, Science and Technology, Japan (since 2010), the Nihon University Multidisciplinary Research Grant (2010–2011), and grants-in-aid for research on hepatitis from the Ministry of Health, Labour and Welfare of Japan . ",

year = "2013",

month = nov,

doi = "10.1016/j.jbiosc.2013.05.006",

language = "English",

volume = "116",

pages = "620--623",

journal = "Journal of Bioscience and Bioengineering",

issn = "1389-1723",

publisher = "The Society for Biotechnology, Japan",

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T1 - Direct targeting of proteins to lipid droplets demonstrated by time-lapse live cell imaging

AU - Tanaka, Torahiko

AU - Kuroda, Kazumichi

AU - Ikeda, Masanori

AU - Kato, Nobuyuki

AU - Shimizu, Kazufumi

AU - Makishima, Makoto

N1 - Funding Information: Technical assistance by K. Toyosawa is gratefully acknowledged. We thank Drs. M. Moriyama and T. Hayashida for valuable comments. This work was supported by Strategic Research Base Development Program for Private Universities subsidized by the Ministry of Education, Culture, Sports, Science and Technology, Japan (since 2010), the Nihon University Multidisciplinary Research Grant (2010–2011), and grants-in-aid for research on hepatitis from the Ministry of Health, Labour and Welfare of Japan .

PY - 2013/11

Y1 - 2013/11

N2 - A protein that specifically targets lipid droplets (LDs) was created by connecting two domains of nonstructural protein 4B containing amphipathic helices from hepatitis C virus. We demonstrated its direct targeting and accumulation to the LD surface by time-lapse live cell imaging, comparable to those observed with adipose differentiation-related protein.

AB - A protein that specifically targets lipid droplets (LDs) was created by connecting two domains of nonstructural protein 4B containing amphipathic helices from hepatitis C virus. We demonstrated its direct targeting and accumulation to the LD surface by time-lapse live cell imaging, comparable to those observed with adipose differentiation-related protein.

KW - Adipose differentiation-related protein

KW - Hepatitis C virus

KW - Lipid droplets

KW - Nonstructural protein 4B

KW - Time-lapse imaging

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JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

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ER -

Direct targeting of proteins to lipid droplets demonstrated by time-lapse live cell imaging

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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