DISC1 localizes to the centrosome by binding to kendrin

Ko Miyoshi; Masato Asanuma; Ikuko Miyazaki; Francisco J. Diaz-Corrales; Taiichi Katayama; Masaya Tohyama; Norio Ogawa

doi:10.1016/j.bbrc.2004.03.163

DISC1 localizes to the centrosome by binding to kendrin

Ko Miyoshi, Masato Asanuma, Ikuko Miyazaki, Francisco J. Diaz-Corrales, Taiichi Katayama, Masaya Tohyama, Norio Ogawa

Research output: Contribution to journal › Article › peer-review

70 Citations (Scopus)

Abstract

Disrupted-In-Schizophrenia 1 (DISC1) was identified as a novel gene disrupted by a (1;11)(q42.1;q14.3) translocation that segregated with major mental disorders in a Scottish family. Using the yeast two-hybrid system, we screened a human brain cDNA library for interactors of the DISC1 protein. One of the positive clones encoded kendrin/pericentrin-B, a giant protein known to localize specifically to the centrosome. The interaction between DISC1 and kendrin in mammalian cells was demonstrated by an immunoprecipitation assay. Residues 446-533 of DISC1 were essential for the interaction with kendrin. Immunocytochemical analysis revealed the colocalization of DISC1 and kendrin to the centrosome. These data indicate that DISC1 localizes to the centrosome by binding to kendrin. Kendrin has been reported to anchor the γ-tubulin complex to the centrosome, providing microtubule nucleation sites. The present study suggests the possible involvement of DISC1 in the pathophysiology of mental disorders due to its putative effect on centrosomal function.

Original language	English
Pages (from-to)	1195-1199
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	317
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2004.03.163
Publication status	Published - May 14 2004

Keywords

Affective disorders
Centrosome
DISC1
Kendrin
Mental disorders
Pericentrin
Schizophrenia

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2004.03.163

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title = "DISC1 localizes to the centrosome by binding to kendrin",

abstract = "Disrupted-In-Schizophrenia 1 (DISC1) was identified as a novel gene disrupted by a (1;11)(q42.1;q14.3) translocation that segregated with major mental disorders in a Scottish family. Using the yeast two-hybrid system, we screened a human brain cDNA library for interactors of the DISC1 protein. One of the positive clones encoded kendrin/pericentrin-B, a giant protein known to localize specifically to the centrosome. The interaction between DISC1 and kendrin in mammalian cells was demonstrated by an immunoprecipitation assay. Residues 446-533 of DISC1 were essential for the interaction with kendrin. Immunocytochemical analysis revealed the colocalization of DISC1 and kendrin to the centrosome. These data indicate that DISC1 localizes to the centrosome by binding to kendrin. Kendrin has been reported to anchor the γ-tubulin complex to the centrosome, providing microtubule nucleation sites. The present study suggests the possible involvement of DISC1 in the pathophysiology of mental disorders due to its putative effect on centrosomal function.",

keywords = "Affective disorders, Centrosome, DISC1, Kendrin, Mental disorders, Pericentrin, Schizophrenia",

author = "Ko Miyoshi and Masato Asanuma and Ikuko Miyazaki and Diaz-Corrales, {Francisco J.} and Taiichi Katayama and Masaya Tohyama and Norio Ogawa",

note = "Funding Information: This work was in part supported by the 21st Century COE program and Grants-in-Aid for Scientific Research (C) and for Young Scientists (B) from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and by Grants-in-Aid for Research on Psychiatric and Neurological Diseases and Mental Health, for Comprehensive Research on Aging and Health, and for Research on Pharmaceutical and Medical Safety from the Ministry of Health, Labour and Welfare of Japan. We thank Mikiko Takahashi and Yoshitaka Ono (Kobe University) for providing the antibody to kendrin.",

year = "2004",

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doi = "10.1016/j.bbrc.2004.03.163",

language = "English",

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journal = "Biochemical and Biophysical Research Communications",

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T1 - DISC1 localizes to the centrosome by binding to kendrin

AU - Miyoshi, Ko

AU - Asanuma, Masato

AU - Miyazaki, Ikuko

AU - Diaz-Corrales, Francisco J.

AU - Katayama, Taiichi

AU - Tohyama, Masaya

AU - Ogawa, Norio

N1 - Funding Information: This work was in part supported by the 21st Century COE program and Grants-in-Aid for Scientific Research (C) and for Young Scientists (B) from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and by Grants-in-Aid for Research on Psychiatric and Neurological Diseases and Mental Health, for Comprehensive Research on Aging and Health, and for Research on Pharmaceutical and Medical Safety from the Ministry of Health, Labour and Welfare of Japan. We thank Mikiko Takahashi and Yoshitaka Ono (Kobe University) for providing the antibody to kendrin.

PY - 2004/5/14

Y1 - 2004/5/14

N2 - Disrupted-In-Schizophrenia 1 (DISC1) was identified as a novel gene disrupted by a (1;11)(q42.1;q14.3) translocation that segregated with major mental disorders in a Scottish family. Using the yeast two-hybrid system, we screened a human brain cDNA library for interactors of the DISC1 protein. One of the positive clones encoded kendrin/pericentrin-B, a giant protein known to localize specifically to the centrosome. The interaction between DISC1 and kendrin in mammalian cells was demonstrated by an immunoprecipitation assay. Residues 446-533 of DISC1 were essential for the interaction with kendrin. Immunocytochemical analysis revealed the colocalization of DISC1 and kendrin to the centrosome. These data indicate that DISC1 localizes to the centrosome by binding to kendrin. Kendrin has been reported to anchor the γ-tubulin complex to the centrosome, providing microtubule nucleation sites. The present study suggests the possible involvement of DISC1 in the pathophysiology of mental disorders due to its putative effect on centrosomal function.

AB - Disrupted-In-Schizophrenia 1 (DISC1) was identified as a novel gene disrupted by a (1;11)(q42.1;q14.3) translocation that segregated with major mental disorders in a Scottish family. Using the yeast two-hybrid system, we screened a human brain cDNA library for interactors of the DISC1 protein. One of the positive clones encoded kendrin/pericentrin-B, a giant protein known to localize specifically to the centrosome. The interaction between DISC1 and kendrin in mammalian cells was demonstrated by an immunoprecipitation assay. Residues 446-533 of DISC1 were essential for the interaction with kendrin. Immunocytochemical analysis revealed the colocalization of DISC1 and kendrin to the centrosome. These data indicate that DISC1 localizes to the centrosome by binding to kendrin. Kendrin has been reported to anchor the γ-tubulin complex to the centrosome, providing microtubule nucleation sites. The present study suggests the possible involvement of DISC1 in the pathophysiology of mental disorders due to its putative effect on centrosomal function.

KW - Affective disorders

KW - Centrosome

KW - DISC1

KW - Kendrin

KW - Mental disorders

KW - Pericentrin

KW - Schizophrenia

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SN - 0006-291X

VL - 317

SP - 1195

EP - 1199

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

DISC1 localizes to the centrosome by binding to kendrin

Abstract

Keywords

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