Dissociation of SNAP-25 and VAMP-2 by MgATP in permeabilized adrenal chromaffin cells

Hiroaki Misonou; Tei ichi Nishiki; Mariko Sekiguchi; Masami Takahashi; Yoichi Kamata; Shunji Kozaki; Mica Ohara-Imaizumi; Konosuke Kumakura

doi:10.1016/0006-8993(96)00954-7

Dissociation of SNAP-25 and VAMP-2 by MgATP in permeabilized adrenal chromaffin cells

Hiroaki Misonou, Tei ichi Nishiki, Mariko Sekiguchi, Masami Takahashi, Yoichi Kamata, Shunji Kozaki, Mica Ohara-Imaizumi, Konosuke Kumakura

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

In digitonin-permeabilized adrenal chromaffin cells, Ca²⁺-induced catecholamine release can be resolved into at least two sequential steps: a MgATP-dependent priming step and a MgATP-independent Ca²⁺-triggered step. Botulinum neurotoxins types A and E cleaved SNAP-25, and blocked MgATP-independent Ca²⁺-induced catecholamine release from the permeabilized chromaffin cells. When the permeabilized cells were primed by pretreatment with MgATP, the amount of SNAP-25 associated with VAMP-2 decreased, and the fraction of SNAP-25 proteolyzed by the neurotoxins increased. These results suggest that dissociation of SNAP-25 and VAMP-2 occurs during the MgATP-dependent priming step, and SNAP-25 plays some important roles in the subsequent MgATP-independent step.

Original language	English
Pages (from-to)	351-355
Number of pages	5
Journal	Brain Research
Volume	737
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(96)00954-7
Publication status	Published - Oct 21 1996
Externally published	Yes

Keywords

Botulinum neurotoxin
Exocytosis
MgATP
SNAP-24
VAMP-2

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

Access to Document

10.1016/0006-8993(96)00954-7

Cite this

@article{9740ccea4fba45d8b4707bfaac056c22,

title = "Dissociation of SNAP-25 and VAMP-2 by MgATP in permeabilized adrenal chromaffin cells",

abstract = "In digitonin-permeabilized adrenal chromaffin cells, Ca2+-induced catecholamine release can be resolved into at least two sequential steps: a MgATP-dependent priming step and a MgATP-independent Ca2+-triggered step. Botulinum neurotoxins types A and E cleaved SNAP-25, and blocked MgATP-independent Ca2+-induced catecholamine release from the permeabilized chromaffin cells. When the permeabilized cells were primed by pretreatment with MgATP, the amount of SNAP-25 associated with VAMP-2 decreased, and the fraction of SNAP-25 proteolyzed by the neurotoxins increased. These results suggest that dissociation of SNAP-25 and VAMP-2 occurs during the MgATP-dependent priming step, and SNAP-25 plays some important roles in the subsequent MgATP-independent step.",

keywords = "Botulinum neurotoxin, Exocytosis, MgATP, SNAP-24, VAMP-2",

author = "Hiroaki Misonou and Nishiki, {Tei ichi} and Mariko Sekiguchi and Masami Takahashi and Yoichi Kamata and Shunji Kozaki and Mica Ohara-Imaizumi and Konosuke Kumakura",

note = "Funding Information: We thank Dr. M.J. Seager for critical reading of the manuscript. This work was performed through Special Coordination Funds of the Science and Technology Agency of the Japanese Government of K.K., and supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, Japan, to M.T.",

year = "1996",

month = oct,

day = "21",

doi = "10.1016/0006-8993(96)00954-7",

language = "English",

volume = "737",

pages = "351--355",

journal = "Brain Research",

issn = "0006-8993",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Dissociation of SNAP-25 and VAMP-2 by MgATP in permeabilized adrenal chromaffin cells

AU - Misonou, Hiroaki

AU - Nishiki, Tei ichi

AU - Sekiguchi, Mariko

AU - Takahashi, Masami

AU - Kamata, Yoichi

AU - Kozaki, Shunji

AU - Ohara-Imaizumi, Mica

AU - Kumakura, Konosuke

N1 - Funding Information: We thank Dr. M.J. Seager for critical reading of the manuscript. This work was performed through Special Coordination Funds of the Science and Technology Agency of the Japanese Government of K.K., and supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, Japan, to M.T.

PY - 1996/10/21

Y1 - 1996/10/21

N2 - In digitonin-permeabilized adrenal chromaffin cells, Ca2+-induced catecholamine release can be resolved into at least two sequential steps: a MgATP-dependent priming step and a MgATP-independent Ca2+-triggered step. Botulinum neurotoxins types A and E cleaved SNAP-25, and blocked MgATP-independent Ca2+-induced catecholamine release from the permeabilized chromaffin cells. When the permeabilized cells were primed by pretreatment with MgATP, the amount of SNAP-25 associated with VAMP-2 decreased, and the fraction of SNAP-25 proteolyzed by the neurotoxins increased. These results suggest that dissociation of SNAP-25 and VAMP-2 occurs during the MgATP-dependent priming step, and SNAP-25 plays some important roles in the subsequent MgATP-independent step.

AB - In digitonin-permeabilized adrenal chromaffin cells, Ca2+-induced catecholamine release can be resolved into at least two sequential steps: a MgATP-dependent priming step and a MgATP-independent Ca2+-triggered step. Botulinum neurotoxins types A and E cleaved SNAP-25, and blocked MgATP-independent Ca2+-induced catecholamine release from the permeabilized chromaffin cells. When the permeabilized cells were primed by pretreatment with MgATP, the amount of SNAP-25 associated with VAMP-2 decreased, and the fraction of SNAP-25 proteolyzed by the neurotoxins increased. These results suggest that dissociation of SNAP-25 and VAMP-2 occurs during the MgATP-dependent priming step, and SNAP-25 plays some important roles in the subsequent MgATP-independent step.

KW - Botulinum neurotoxin

KW - Exocytosis

KW - MgATP

KW - SNAP-24

KW - VAMP-2

UR - http://www.scopus.com/inward/record.url?scp=0030597143&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030597143&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(96)00954-7

DO - 10.1016/0006-8993(96)00954-7

M3 - Article

C2 - 8930391

AN - SCOPUS:0030597143

SN - 0006-8993

VL - 737

SP - 351

EP - 355

JO - Brain Research

JF - Brain Research

IS - 1-2

ER -

Dissociation of SNAP-25 and VAMP-2 by MgATP in permeabilized adrenal chromaffin cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this