DNA strand breaks induced by ionizing radiation on Rubrobacter radiotolerans, an extremely radioresistant bacterium

Hiroaki Terato; Mutsumi Kobayashi; Osamu Yamamoto; Hiroshi Ide

doi:10.1016/S0944-5013(99)80011-1

DNA strand breaks induced by ionizing radiation on Rubrobacter radiotolerans, an extremely radioresistant bacterium

Hiroaki Terato, Mutsumi Kobayashi, Osamu Yamamoto, Hiroshi Ide

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Rubrobacter radiotoIerans is the most radioresistant bacterium showing 16kGy as D37 against gamma-rays. However mechanisms of the radioresistance have been still unclear. To clarify the post-irradiating events in the cell, we investigated DNA strand breaks which is the most major lesion induced by ionizing radiation. The neutral sucrose density gradient centrifugation analysis showed that size of the chromosomal DNA gradually decreased with irradiation dose. However, the frequency of DNA strand breaks after ionizing irradiation was significantly lower than those reported for other eubacteria. The reduced DNA sizes were not recovered during the postirradiating cultivation. These results suggest that in this organism DNA protection from damage is mainly involved in the radioresistance, but not DNA repair activities.

Original language	English
Pages (from-to)	173-178
Number of pages	6
Journal	Microbiological Research
Volume	154
Issue number	2
DOIs	https://doi.org/10.1016/S0944-5013(99)80011-1
Publication status	Published - Sept 1999
Externally published	Yes

Keywords

DNA damage
Free radical
Radiation
Radioresistance
Rubrobacter radiotolerans

ASJC Scopus subject areas

Microbiology

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10.1016/S0944-5013(99)80011-1

Cite this

@article{0eb93fdba83f439a89d3c246b5444102,

title = "DNA strand breaks induced by ionizing radiation on Rubrobacter radiotolerans, an extremely radioresistant bacterium",

abstract = "Rubrobacter radiotoIerans is the most radioresistant bacterium showing 16kGy as D37 against gamma-rays. However mechanisms of the radioresistance have been still unclear. To clarify the post-irradiating events in the cell, we investigated DNA strand breaks which is the most major lesion induced by ionizing radiation. The neutral sucrose density gradient centrifugation analysis showed that size of the chromosomal DNA gradually decreased with irradiation dose. However, the frequency of DNA strand breaks after ionizing irradiation was significantly lower than those reported for other eubacteria. The reduced DNA sizes were not recovered during the postirradiating cultivation. These results suggest that in this organism DNA protection from damage is mainly involved in the radioresistance, but not DNA repair activities.",

keywords = "DNA damage, Free radical, Radiation, Radioresistance, Rubrobacter radiotolerans",

author = "Hiroaki Terato and Mutsumi Kobayashi and Osamu Yamamoto and Hiroshi Ide",

note = "Funding Information: This work was supported by Showa-Shell Foundation, Japan. The authors thank Dr. Nobuaki Ohta (Hiroshima University) for using 6OCo gamma-ray source, and Yosuke Baba and Miho Suzuki (Hiroshima University) for preliminary works.",

year = "1999",

month = sep,

doi = "10.1016/S0944-5013(99)80011-1",

language = "English",

volume = "154",

pages = "173--178",

journal = "Microbiological Research",

issn = "0944-5013",

publisher = "Urban und Fischer Verlag Jena",

number = "2",

}

TY - JOUR

T1 - DNA strand breaks induced by ionizing radiation on Rubrobacter radiotolerans, an extremely radioresistant bacterium

AU - Terato, Hiroaki

AU - Kobayashi, Mutsumi

AU - Yamamoto, Osamu

AU - Ide, Hiroshi

N1 - Funding Information: This work was supported by Showa-Shell Foundation, Japan. The authors thank Dr. Nobuaki Ohta (Hiroshima University) for using 6OCo gamma-ray source, and Yosuke Baba and Miho Suzuki (Hiroshima University) for preliminary works.

PY - 1999/9

Y1 - 1999/9

N2 - Rubrobacter radiotoIerans is the most radioresistant bacterium showing 16kGy as D37 against gamma-rays. However mechanisms of the radioresistance have been still unclear. To clarify the post-irradiating events in the cell, we investigated DNA strand breaks which is the most major lesion induced by ionizing radiation. The neutral sucrose density gradient centrifugation analysis showed that size of the chromosomal DNA gradually decreased with irradiation dose. However, the frequency of DNA strand breaks after ionizing irradiation was significantly lower than those reported for other eubacteria. The reduced DNA sizes were not recovered during the postirradiating cultivation. These results suggest that in this organism DNA protection from damage is mainly involved in the radioresistance, but not DNA repair activities.

AB - Rubrobacter radiotoIerans is the most radioresistant bacterium showing 16kGy as D37 against gamma-rays. However mechanisms of the radioresistance have been still unclear. To clarify the post-irradiating events in the cell, we investigated DNA strand breaks which is the most major lesion induced by ionizing radiation. The neutral sucrose density gradient centrifugation analysis showed that size of the chromosomal DNA gradually decreased with irradiation dose. However, the frequency of DNA strand breaks after ionizing irradiation was significantly lower than those reported for other eubacteria. The reduced DNA sizes were not recovered during the postirradiating cultivation. These results suggest that in this organism DNA protection from damage is mainly involved in the radioresistance, but not DNA repair activities.

KW - DNA damage

KW - Free radical

KW - Radiation

KW - Radioresistance

KW - Rubrobacter radiotolerans

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U2 - 10.1016/S0944-5013(99)80011-1

DO - 10.1016/S0944-5013(99)80011-1

M3 - Article

AN - SCOPUS:0032879979

SN - 0944-5013

VL - 154

SP - 173

EP - 178

JO - Microbiological Research

JF - Microbiological Research

IS - 2

ER -

DNA strand breaks induced by ionizing radiation on Rubrobacter radiotolerans, an extremely radioresistant bacterium

Abstract

Keywords

ASJC Scopus subject areas

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