@article{35ba7c107fc843f9ae4ef7baf1700c84,
title = "Dorsal clock networks drive temperature preference rhythms in Drosophila",
abstract = "Animals display a body temperature rhythm (BTR). Little is known about the mechanisms by which a rhythmic pattern of BTR is regulated and how body temperature is set at different times of the day. As small ectotherms, Drosophila exhibit a daily temperature preference rhythm (TPR), which generates BTR. Here, we demonstrate dorsal clock networks that play essential roles in TPR. Dorsal neurons 2 (DN2s) are the main clock for TPR. We find that DN2s and posterior DN1s (DN1ps) contact and the extent of contacts increases during the day and that the silencing of DN2s or DN1ps leads to a lower temperature preference. The data suggest that temporal control of the microcircuit from DN2s to DN1ps contributes to TPR regulation. We also identify anterior DN1s (DN1as) as another important clock for TPR. Thus, we show that the DN networks predominantly control TPR and determine both a rhythmic pattern and preferred temperatures.",
keywords = "anterior dorsal neurons 1, body temperature rhythms and Drosophila melanogaster, circadian clock, circadian rhythms, CP: Neuroscience, dorsal neurons 2, posterior dorsal neurons 1, temperature homeostasis, temperature preference rhythms, thermoregulation",
author = "Chen, {Shyh Chi} and Xin Tang and Tadahiro Goda and Yujiro Umezaki and Riley, {Abigail C.} and Manabu Sekiguchi and Taishi Yoshii and Hamada, {Fumika N.}",
note = "Funding Information: We are grateful to Drs. Kristin Scott, Orie Shafer, Mimi Shirasu-Hiza, Leslie Griffith, Gerald M. Rubin, Aljoscha Nern, and Guillermo Gonzalez, the Bloomington Drosophila fly stock center, the Vienna Drosophila RNAi Center, and the Developmental Studies Hybridoma Bank for providing the fly lines; Dr. Paul Hardin for providing the antibodies; and the Hamada lab members for their comments and advice on the manuscript. This research was supported by JSPS KAKENHI grant 19H03265 to T.Y. RIP funding, the Charlotte R. Schmidlapp Women Scholars Program of Cincinnati Children's Hospital, and NIH R01 grant GM107582 to F.N.H. and R21 grant NS112890 to F.N.H. Conceptualization, F.N.H. S.C.C. and X.T.; Methodology, F.N.H. S.C.C. and X.T.; Investigation, S.C.C. X.T. T.G. U.Y. A.C.R. and M.S.; Writing – Original Draft, F.N.H.; Writing – Review & Editing, S.C.C. X.T. and T.G.; Funding Acquisition, F.N.H. and Y.T.; Visualization, F.N.H. S.C.C. X.T. and T.G.; Resources, S.C.C. X.T. T.G. U.Y. A.C.R. and M.S.; Supervision, F.N.H. and Y.T. The authors declare no competing interests. Funding Information: We are grateful to Drs. Kristin Scott, Orie Shafer, Mimi Shirasu-Hiza, Leslie Griffith, Gerald M. Rubin, Aljoscha Nern, and Guillermo Gonzalez, the Bloomington Drosophila fly stock center, the Vienna Drosophila RNAi Center, and the Developmental Studies Hybridoma Bank for providing the fly lines; Dr. Paul Hardin for providing the antibodies; and the Hamada lab members for their comments and advice on the manuscript. This research was supported by JSPS KAKENHI grant 19H03265 to T.Y., RIP funding, the Charlotte R. Schmidlapp Women Scholars Program of Cincinnati Children{\textquoteright}s Hospital, and NIH R01 grant GM107582 to F.N.H. and R21 grant NS112890 to F.N.H. Publisher Copyright: {\textcopyright} 2022 The Authors",
year = "2022",
month = apr,
day = "12",
doi = "10.1016/j.celrep.2022.110668",
language = "English",
volume = "39",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "2",
}