TY - JOUR
T1 - Duodenal follicular lymphoma lacks AID but expresses BACH2 and has memory B-cell characteristics
AU - Takata, Katsuyoshi
AU - Sato, Yasuharu
AU - Nakamura, Naoya
AU - Tokunaka, Mami
AU - Miki, Yukari
AU - Yukie Kikuti, Yara
AU - Igarashi, Kazuhiko
AU - Ito, Etsuro
AU - Harigae, Hideo
AU - Kato, Seiichi
AU - Hayashi, Eiko
AU - Oka, Takashi
AU - Hoshii, Yoshinobu
AU - Tari, Akira
AU - Okada, Hiroyuki
AU - Mohamad, Abd Alkader Lamia
AU - Maeda, Yoshinobu
AU - Tanimoto, Mitsune
AU - Kinoshita, Tomohiro
AU - Yoshino, Tadashi
N1 - Funding Information:
This work was supported in part by grants from the Japan Society for the Promotion Science (JSPS no. 19590348) and was supported in part by a Grant-in-Aid for Cancer Research (21-6-3) from the Ministry of Health, Labor and Welfare, Tokyo, Japan. We special thanks Ms H Nakamura, Ms M Okabe, Dr T Kunitomo, Dr A Uchiyama, Dr S Nose and Dr T Miyake for their technical assistance and preparing pathological samples.
PY - 2013/1
Y1 - 2013/1
N2 - We have reported previously that duodenal follicular lymphoma (FL) is distinct from nodal FL and showed more resemblance to mucosa-associated lymphoid tissue lymphoma, and that FL frequently involved the duodenal second portion. In the present study, we examined duodenal FLs and gastric/colonic FLs to clarify the clinicopathological and immunological differences between the tumor types. We analyzed 8 samples of gastric FL, 17 of duodenal ones, and 5 of colonic/rectal ones, and characterized them by immunohistochemistry, immunogenotyping, and histology. Gastric and colonic FLs presented in submucosal to subserosal areas, whereas duodenal ones presented in the mucosal to submucosal layers. Immunohistochemical analysis revealed that duodenal FLs exhibited the following phenotypes: CD10 (+), B-cell lymphoma 2 (BCL-2) (+), BCL-6 (+), activation-induced cytidine deaminase (AID) (-), BACH2 (+), CD27 (+), MUM-1 (-), Blimp-1 (-), and loose CD21 network (duodenal pattern). Gastric/colonic FLs exhibited the following phenotypes: CD10 (+), BCL-2 (+), BCL-6 (+), AID (+), BACH2 (+), CD27 (-), MUM-1 (-), Blimp-1 (-), and a dense CD21 network (nodal pattern). Expression of AID and CD27 in lymphoma cells and the CD21 network pattern were considerably different between duodenal FLs and gastric/colonic ones. Moreover, in situ hybridization revealed that, in the duodenal FLs, BACH2 was expressed at the periphery of the tumor follicle and tumor villi. The number of immunoglobulin heavy-chain variable domains VH4 and VH5 were higher in duodenal follicular lymphomoas than in gastric FLs. The lymphoma cells of duodenal FLs are different from those of gastric/colonic FLs, and duodenal FL is distinct even within the gastrointestinal tract. Somatic hypermutation in immunoglobulin genes and CD27 expression are hallmarks of memory B cells. We suggest that duodenal FL cells are in the memory B-cell stage, and require BACH2 instead of AID for ongoing mutation.
AB - We have reported previously that duodenal follicular lymphoma (FL) is distinct from nodal FL and showed more resemblance to mucosa-associated lymphoid tissue lymphoma, and that FL frequently involved the duodenal second portion. In the present study, we examined duodenal FLs and gastric/colonic FLs to clarify the clinicopathological and immunological differences between the tumor types. We analyzed 8 samples of gastric FL, 17 of duodenal ones, and 5 of colonic/rectal ones, and characterized them by immunohistochemistry, immunogenotyping, and histology. Gastric and colonic FLs presented in submucosal to subserosal areas, whereas duodenal ones presented in the mucosal to submucosal layers. Immunohistochemical analysis revealed that duodenal FLs exhibited the following phenotypes: CD10 (+), B-cell lymphoma 2 (BCL-2) (+), BCL-6 (+), activation-induced cytidine deaminase (AID) (-), BACH2 (+), CD27 (+), MUM-1 (-), Blimp-1 (-), and loose CD21 network (duodenal pattern). Gastric/colonic FLs exhibited the following phenotypes: CD10 (+), BCL-2 (+), BCL-6 (+), AID (+), BACH2 (+), CD27 (-), MUM-1 (-), Blimp-1 (-), and a dense CD21 network (nodal pattern). Expression of AID and CD27 in lymphoma cells and the CD21 network pattern were considerably different between duodenal FLs and gastric/colonic ones. Moreover, in situ hybridization revealed that, in the duodenal FLs, BACH2 was expressed at the periphery of the tumor follicle and tumor villi. The number of immunoglobulin heavy-chain variable domains VH4 and VH5 were higher in duodenal follicular lymphomoas than in gastric FLs. The lymphoma cells of duodenal FLs are different from those of gastric/colonic FLs, and duodenal FL is distinct even within the gastrointestinal tract. Somatic hypermutation in immunoglobulin genes and CD27 expression are hallmarks of memory B cells. We suggest that duodenal FL cells are in the memory B-cell stage, and require BACH2 instead of AID for ongoing mutation.
KW - BACH2
KW - gastrointestinal follicular lymphoma
KW - memory B cell
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U2 - 10.1038/modpathol.2012.127
DO - 10.1038/modpathol.2012.127
M3 - Article
C2 - 22899287
AN - SCOPUS:84871927234
SN - 0893-3952
VL - 26
SP - 22
EP - 31
JO - Modern Pathology
JF - Modern Pathology
IS - 1
ER -