Duplication of acetylcholinesterase gene in diamondback moth strains with different sensitivities to acephate

Shoji Sonoda; Xueyan Shi; Dunlun Song; Pei Liang; Xiwu Gao; Youjun Zhang; Jianhong Li; Yong Liu; Ming Li; Masaya Matsumura; Sachiyo Sanada-Morimura; Chieka Minakuchi; Toshiharu Tanaka; Tadashi Miyata

doi:10.1016/j.ibmb.2014.02.008

Duplication of acetylcholinesterase gene in diamondback moth strains with different sensitivities to acephate

Shoji Sonoda, Xueyan Shi, Dunlun Song, Pei Liang, Xiwu Gao, Youjun Zhang, Jianhong Li, Yong Liu, Ming Li, Masaya Matsumura, Sachiyo Sanada-Morimura, Chieka Minakuchi, Toshiharu Tanaka, Tadashi Miyata

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12 Citations (Scopus)

Abstract

This study examined the acetylcholinesterase 1 gene (AChE1) in Plutella xylostella strains with different sensitivities to acephate. Multiple haplotypes of the gene were found in the field-collected strains including distinct haplotypes carrying one or both previously reported mutations (A298S and G324A). Moreover, sequencing results indicated the presence of duplicated copies of the gene in the field-collected strains. No correlation was found between copy numbers of AChE1 and levels of resistance to acephate suggesting that extensive AChE1 duplication is not a major resistance factor at least in some P.xylostella strains. Proportions of the A298S and G324A mutations showed no correlation with levels of resistance to acephate. This suggests that acephate resistance of P.xylostella is complex and cannot be evaluated based on the AChE1 copy number or proportions of the resistance mutations alone.

Original language	English
Pages (from-to)	83-90
Number of pages	8
Journal	Insect Biochemistry and Molecular Biology
Volume	48
Issue number	1
DOIs	https://doi.org/10.1016/j.ibmb.2014.02.008
Publication status	Published - May 2014

Keywords

Acetylcholinesterase
Diamondback moth
Gene duplication
Mutation
Organophosphate

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Insect Science

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10.1016/j.ibmb.2014.02.008

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Sonoda, S., Shi, X., Song, D., Liang, P., Gao, X., Zhang, Y., Li, J., Liu, Y., Li, M., Matsumura, M., Sanada-Morimura, S., Minakuchi, C., Tanaka, T., & Miyata, T. (2014). Duplication of acetylcholinesterase gene in diamondback moth strains with different sensitivities to acephate. Insect Biochemistry and Molecular Biology, 48(1), 83-90. https://doi.org/10.1016/j.ibmb.2014.02.008

Sonoda, S, Shi, X, Song, D, Liang, P, Gao, X, Zhang, Y, Li, J, Liu, Y, Li, M, Matsumura, M, Sanada-Morimura, S, Minakuchi, C, Tanaka, T & Miyata, T 2014, 'Duplication of acetylcholinesterase gene in diamondback moth strains with different sensitivities to acephate', Insect Biochemistry and Molecular Biology, vol. 48, no. 1, pp. 83-90. https://doi.org/10.1016/j.ibmb.2014.02.008

@article{56550d915d2b42eea3c9ef5fdfb63f1f,

title = "Duplication of acetylcholinesterase gene in diamondback moth strains with different sensitivities to acephate",

abstract = "This study examined the acetylcholinesterase 1 gene (AChE1) in Plutella xylostella strains with different sensitivities to acephate. Multiple haplotypes of the gene were found in the field-collected strains including distinct haplotypes carrying one or both previously reported mutations (A298S and G324A). Moreover, sequencing results indicated the presence of duplicated copies of the gene in the field-collected strains. No correlation was found between copy numbers of AChE1 and levels of resistance to acephate suggesting that extensive AChE1 duplication is not a major resistance factor at least in some P.xylostella strains. Proportions of the A298S and G324A mutations showed no correlation with levels of resistance to acephate. This suggests that acephate resistance of P.xylostella is complex and cannot be evaluated based on the AChE1 copy number or proportions of the resistance mutations alone.",

keywords = "Acetylcholinesterase, Diamondback moth, Gene duplication, Mutation, Organophosphate",

author = "Shoji Sonoda and Xueyan Shi and Dunlun Song and Pei Liang and Xiwu Gao and Youjun Zhang and Jianhong Li and Yong Liu and Ming Li and Masaya Matsumura and Sachiyo Sanada-Morimura and Chieka Minakuchi and Toshiharu Tanaka and Tadashi Miyata",

note = "Funding Information: This study was supported in part by a Grant-in-Aid for Scientific Research (C) from the Japan Society for the Promotion of Science (JSPS) (No. 23580072 ), the JSPS – National Natural Science Foundation of China (NSFC) Joint Research Project, and the Ohara Foundation. The authors thank Ito, K., Sakurai, T., Higuchi, H., Honda, K., Matsukura, K. (National Agriculture and Food Research Organization), Shimizu, K.-I., Shimizu, K. (Chiba Prefecture, Japan), Morishita, M., Iguchi, M. (Wakayama Prefecture, Japan), Shibao, M. (Osaka Prefecture, Japan), Gyoutoku, Y. (Kumamoto Prefecture, Japan), Narai, Y. (Shimane Prefecture, Japan), Sukontsbhirom, S. (Department of Agriculture, Thailand), Rushtapakonchai, W. (Thep Wantana Chemical Co. Ltd., Thailand), and Wu, G. (Fujian Agriculture and Forestry University, China) for insect collection. The authors also thank Noda, H. (National Institute of Agrobiological Science, Japan) for reading the manuscript and Yamashita, J., Kohara, Y. and Kataoka, Y. (IPSR, Okayama University, Japan) for their assistance. Appendix A ",

year = "2014",

month = may,

doi = "10.1016/j.ibmb.2014.02.008",

language = "English",

volume = "48",

pages = "83--90",

journal = "Insect Biochemistry and Molecular Biology",

issn = "0965-1748",

publisher = "Elsevier Limited",

number = "1",

}

TY - JOUR

T1 - Duplication of acetylcholinesterase gene in diamondback moth strains with different sensitivities to acephate

AU - Sonoda, Shoji

AU - Shi, Xueyan

AU - Song, Dunlun

AU - Liang, Pei

AU - Gao, Xiwu

AU - Zhang, Youjun

AU - Li, Jianhong

AU - Liu, Yong

AU - Li, Ming

AU - Matsumura, Masaya

AU - Sanada-Morimura, Sachiyo

AU - Minakuchi, Chieka

AU - Tanaka, Toshiharu

AU - Miyata, Tadashi

N1 - Funding Information: This study was supported in part by a Grant-in-Aid for Scientific Research (C) from the Japan Society for the Promotion of Science (JSPS) (No. 23580072 ), the JSPS – National Natural Science Foundation of China (NSFC) Joint Research Project, and the Ohara Foundation. The authors thank Ito, K., Sakurai, T., Higuchi, H., Honda, K., Matsukura, K. (National Agriculture and Food Research Organization), Shimizu, K.-I., Shimizu, K. (Chiba Prefecture, Japan), Morishita, M., Iguchi, M. (Wakayama Prefecture, Japan), Shibao, M. (Osaka Prefecture, Japan), Gyoutoku, Y. (Kumamoto Prefecture, Japan), Narai, Y. (Shimane Prefecture, Japan), Sukontsbhirom, S. (Department of Agriculture, Thailand), Rushtapakonchai, W. (Thep Wantana Chemical Co. Ltd., Thailand), and Wu, G. (Fujian Agriculture and Forestry University, China) for insect collection. The authors also thank Noda, H. (National Institute of Agrobiological Science, Japan) for reading the manuscript and Yamashita, J., Kohara, Y. and Kataoka, Y. (IPSR, Okayama University, Japan) for their assistance. Appendix A

PY - 2014/5

Y1 - 2014/5

N2 - This study examined the acetylcholinesterase 1 gene (AChE1) in Plutella xylostella strains with different sensitivities to acephate. Multiple haplotypes of the gene were found in the field-collected strains including distinct haplotypes carrying one or both previously reported mutations (A298S and G324A). Moreover, sequencing results indicated the presence of duplicated copies of the gene in the field-collected strains. No correlation was found between copy numbers of AChE1 and levels of resistance to acephate suggesting that extensive AChE1 duplication is not a major resistance factor at least in some P.xylostella strains. Proportions of the A298S and G324A mutations showed no correlation with levels of resistance to acephate. This suggests that acephate resistance of P.xylostella is complex and cannot be evaluated based on the AChE1 copy number or proportions of the resistance mutations alone.

AB - This study examined the acetylcholinesterase 1 gene (AChE1) in Plutella xylostella strains with different sensitivities to acephate. Multiple haplotypes of the gene were found in the field-collected strains including distinct haplotypes carrying one or both previously reported mutations (A298S and G324A). Moreover, sequencing results indicated the presence of duplicated copies of the gene in the field-collected strains. No correlation was found between copy numbers of AChE1 and levels of resistance to acephate suggesting that extensive AChE1 duplication is not a major resistance factor at least in some P.xylostella strains. Proportions of the A298S and G324A mutations showed no correlation with levels of resistance to acephate. This suggests that acephate resistance of P.xylostella is complex and cannot be evaluated based on the AChE1 copy number or proportions of the resistance mutations alone.

KW - Acetylcholinesterase

KW - Diamondback moth

KW - Gene duplication

KW - Mutation

KW - Organophosphate

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U2 - 10.1016/j.ibmb.2014.02.008

DO - 10.1016/j.ibmb.2014.02.008

M3 - Article

C2 - 24632376

AN - SCOPUS:84897008124

SN - 0965-1748

VL - 48

SP - 83

EP - 90

JO - Insect Biochemistry and Molecular Biology

JF - Insect Biochemistry and Molecular Biology

IS - 1

ER -

Duplication of acetylcholinesterase gene in diamondback moth strains with different sensitivities to acephate

Abstract

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