Dynamic aspects of ovarian superoxide dismutase isozymes during the ovulatory process in the rat

Eisuke F. Sato; Hirotsugu Kobuchi; Keisuke Edashige; Masahiko Takahashi; Tamotsu Yoshioka; Kozo Utsumi; Masayasu Inoue

doi:10.1016/0014-5793(92)80502-8

Dynamic aspects of ovarian superoxide dismutase isozymes during the ovulatory process in the rat

Eisuke F. Sato, Hirotsugu Kobuchi, Keisuke Edashige, Masahiko Takahashi, Tamotsu Yoshioka, Kozo Utsumi, Masayasu Inoue

Research output: Contribution to journal › Article › peer-review

73 Citations (Scopus)

Abstract

To investigate the role of superoxide dismutase (SOD) in the ovulatory process, SOD isozymes and their mRNAs were determined in the ovary of 22-day-old rat. After treatment with pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG), ovarian activity of Mn-SOD decreased markedly while Cu/Zn-SOD remained unchanged. However, the ovarian level of mRNA for Mn-SOD markedly increased after hCG-treatment while that for Cu/Zn-SOD decreased only slightly. Ovulation was inhibited by intravenous injection of a long-acting SOD. These results suggested that superoxide radicals in the ovary might play a critical role in the mechanism for hCG-induced ovulation.

Original language	English
Pages (from-to)	121-125
Number of pages	5
Journal	FEBS Letters
Volume	303
Issue number	2-3
DOIs	https://doi.org/10.1016/0014-5793(92)80502-8
Publication status	Published - Jun 1 1992
Externally published	Yes

Keywords

Cu/Zn-SOD
Mn-SOD
Ovulation
Rat ovary
Superoxide
mRNA

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(92)80502-8

Cite this

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abstract = "To investigate the role of superoxide dismutase (SOD) in the ovulatory process, SOD isozymes and their mRNAs were determined in the ovary of 22-day-old rat. After treatment with pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG), ovarian activity of Mn-SOD decreased markedly while Cu/Zn-SOD remained unchanged. However, the ovarian level of mRNA for Mn-SOD markedly increased after hCG-treatment while that for Cu/Zn-SOD decreased only slightly. Ovulation was inhibited by intravenous injection of a long-acting SOD. These results suggested that superoxide radicals in the ovary might play a critical role in the mechanism for hCG-induced ovulation.",

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AU - Sato, Eisuke F.

AU - Kobuchi, Hirotsugu

AU - Edashige, Keisuke

AU - Takahashi, Masahiko

AU - Yoshioka, Tamotsu

AU - Utsumi, Kozo

AU - Inoue, Masayasu

PY - 1992/6/1

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N2 - To investigate the role of superoxide dismutase (SOD) in the ovulatory process, SOD isozymes and their mRNAs were determined in the ovary of 22-day-old rat. After treatment with pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG), ovarian activity of Mn-SOD decreased markedly while Cu/Zn-SOD remained unchanged. However, the ovarian level of mRNA for Mn-SOD markedly increased after hCG-treatment while that for Cu/Zn-SOD decreased only slightly. Ovulation was inhibited by intravenous injection of a long-acting SOD. These results suggested that superoxide radicals in the ovary might play a critical role in the mechanism for hCG-induced ovulation.

AB - To investigate the role of superoxide dismutase (SOD) in the ovulatory process, SOD isozymes and their mRNAs were determined in the ovary of 22-day-old rat. After treatment with pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG), ovarian activity of Mn-SOD decreased markedly while Cu/Zn-SOD remained unchanged. However, the ovarian level of mRNA for Mn-SOD markedly increased after hCG-treatment while that for Cu/Zn-SOD decreased only slightly. Ovulation was inhibited by intravenous injection of a long-acting SOD. These results suggested that superoxide radicals in the ovary might play a critical role in the mechanism for hCG-induced ovulation.

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KW - Ovulation

KW - Rat ovary

KW - Superoxide

KW - mRNA

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Dynamic aspects of ovarian superoxide dismutase isozymes during the ovulatory process in the rat

Abstract

Keywords

ASJC Scopus subject areas

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