TY - JOUR
T1 - Effect of dentin depth on hybridization quality using different bonding tactics in vivo
AU - Yuan, Y.
AU - Shimada, Y.
AU - Ichinose, S.
AU - Tagami, J.
N1 - Funding Information:
The research was partly supported by 21st Century Center of Excellence (COE) Program for Frontier Research on Molecular Destruction and Reconstruction of Tooth and Bone, Tokyo Medical and Dental University, Tokyo, Japan. The assistance of Mr. Masaomi Ikeda, regarding statistical analysis and Dr. Alireza Sadr is greatly appreciated. We also wish to thank 3M ESPE Company and Kuraray Medical for providing the resin materials.
PY - 2007/8
Y1 - 2007/8
N2 - Objectives: Incomplete resin infiltration and polymerization of adhesive contributed to nanoleakage formation. This study tested the null hypothesis that adoption of different bonding tactics and dentine depth will not affect hybridization quality in vivo. Methods: Class V cavities were prepared on the labial/buccal surface of monkey teeth. They were bonded by Single Bond (a two-step total-etch adhesive), Clearfil SE Bond (a two-step self-etch adhesive), or Clearfil S3 Bond (an all-in-one self-etch adhesive). Combined nanoleakage analysis and quantitative immunolabeling evaluation were carried out in the hybrid layer formed in both cervical superficial and deep dentine. Results: Single Bond showed reticular and spotted nanoleakage while Clearfil SE Bond and Clearfil S3 Bond presented only a spotted one. While Single Bond showed increased concentration of labeling of type I collagen within the deep part of the hybrid layer, two self-etch adhesives-Clearfil SE Bond and Clearfil S3 Bond revealed a homogeneous labeling pattern, even if the latter presented a significantly increased labeling index in deep dentine. Conclusions: Different bonding tactics showed different nanoleakage patterns and immunolabeling index, and was influenced by dentine depth at different levels in vivo.
AB - Objectives: Incomplete resin infiltration and polymerization of adhesive contributed to nanoleakage formation. This study tested the null hypothesis that adoption of different bonding tactics and dentine depth will not affect hybridization quality in vivo. Methods: Class V cavities were prepared on the labial/buccal surface of monkey teeth. They were bonded by Single Bond (a two-step total-etch adhesive), Clearfil SE Bond (a two-step self-etch adhesive), or Clearfil S3 Bond (an all-in-one self-etch adhesive). Combined nanoleakage analysis and quantitative immunolabeling evaluation were carried out in the hybrid layer formed in both cervical superficial and deep dentine. Results: Single Bond showed reticular and spotted nanoleakage while Clearfil SE Bond and Clearfil S3 Bond presented only a spotted one. While Single Bond showed increased concentration of labeling of type I collagen within the deep part of the hybrid layer, two self-etch adhesives-Clearfil SE Bond and Clearfil S3 Bond revealed a homogeneous labeling pattern, even if the latter presented a significantly increased labeling index in deep dentine. Conclusions: Different bonding tactics showed different nanoleakage patterns and immunolabeling index, and was influenced by dentine depth at different levels in vivo.
KW - Dentin depth
KW - FE-SEM
KW - Monoclonal type I collagen antibody
KW - Nanoleakage
KW - TEM
KW - YAG backscattered electron
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U2 - 10.1016/j.jdent.2007.05.002
DO - 10.1016/j.jdent.2007.05.002
M3 - Article
C2 - 17574319
AN - SCOPUS:34447096549
SN - 0300-5712
VL - 35
SP - 664
EP - 672
JO - Journal of Dentistry
JF - Journal of Dentistry
IS - 8
ER -