Effect of diglucosamine on the entrapment of protein into liposomes

S. Murakami, T. Ono, S. Sakai, H. Ijima, K. Kawakami

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


Liposomes, which had entrapped bovine serum albumin (BSA), were modified with diglucosamine by two methods. The liposome was prepared by a freeze-thawing method in the presence of the disaccharide, or the disaccharide was added to the liposome prepared in advance without it. To examine the effects of diglucosamine, the morphology, mean particle size, and zeta potential of both liposomes were compared with those of BSA-entrapping liposome prepared without the disaccharide. Diglucosamine caused no remarkable change in shape and no aggregation of the liposome. The presence of the disaccharide was confirmed on the surfaces of modified liposomes, and the entrapment of BSA into the liposomes was increased by the disaccharide. The entrapment behavior was affected by the way the disaccharide was added, and the difference in the way the BSA was entrapped was also indicated.

Original languageEnglish
Pages (from-to)103-112
Number of pages10
JournalJournal of Liposome Research
Issue number2
Publication statusPublished - Jul 2006
Externally publishedYes


  • Bovine serum albumin
  • Diglucosamine
  • Entrapment
  • Freeze-thawing method
  • Liposomes

ASJC Scopus subject areas

  • Pharmaceutical Science


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