Effect of endotoxin-induced reactive oxygen species on sperm motility

Kenichiro Urata, Hisashi Narahara, Yuichiro Tanaka, Toru Egashira, Fusako Takayama, Isao Miyakawa

Research output: Contribution to journalArticlepeer-review

86 Citations (Scopus)


Objective: To define the mechanism of infection-induced damage of sperm. Design: The effect of lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) on sperm motility and its modification by scavengers were investigated. Setting: Research laboratory of a university hospital. Patient(s): Normozoospermic semen samples were obtained from 37 healthy volunteers. Intervention(s): The sperms were incubated in the presence of LPS with or without scavengers. Main Outcome Measure(s): Sperm motility was evaluated by a sperm quality analyzer (SQAIIB). ROS formation in semen samples was measured by a Berthold luminometer (LB953). Result(s): Motility of spermatozoa was decreased in the LPS-treated samples compared with that in the control groups. ROS was significantly higher in the LPS-treated groups than in the control groups. The addition of ROS scavengers restored the motility index and suppressed ROS production in the LPS-treated semen samples. Conclusion(s): These data suggest that endotoxin-induced excessive production of ROS is responsible for the decrease in sperm motility and that antioxidant therapy may be a therapeutic option for infertile men with bacterial genital tract infection.

Original languageEnglish
Pages (from-to)163-166
Number of pages4
JournalFertility and Sterility
Issue number1
Publication statusPublished - 2001
Externally publishedYes


  • Lipopolysaccharide
  • Reactive oxygen species
  • Scavengers
  • Sperm
  • Sperm motility

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology


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