Effect of fluoride-substituted apatite on in vivo bone formation

Miho Inoue, Andrea P. Rodriguez, Noriyuki Nagai, Hitoshi Nagatsuka, Racquel Z. Legeros, Hidetsugu Tsujigiwa, Masahisa Inoue, Etsuo Kishimoto, Shin Takagi

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)


Biological apatites are characterized by the presence of minor constituents such as magnesium (Mg), chloride (Cl), or fluoride (F) ions. These ions affect cell proliferation and osteoblastic differentiation during bone tissue formation. F-substituted apatites are being explored as potential bonegraft materials. The aim of the present study is to investigate the mechanism of bone formation induced by fluoride-substituted apatite (FAp) by analyzing the effect of FAp on the process of in vivo bone formation. FAps containing different F concentrations (l-FAp: 0.48wt%, m-FAp: 0.91wt%, h-FAp: 2.23wt%) and calcium-deficient apatite (CDA), as positive control, were implanted in rat tibia and bone formation was evaluated by histological examination, immuhistochemistry, in situ hybridization and tartrate-resistant acid phosphatase examinations. The results showed that l-FAp, m-FAp, h-FAp, and CDA biomaterials allowed migration of macrophages, attachment, proliferation, and phenotypic expression of bone cells leading to new bone formation in direct apposition to the particles. However, the l-FAp preparation allowed faster bone conduction compared to the other experimental materials. These results suggest that FAp with low F concentration may be an efficient bonegraft material for dental and medical application.

Original languageEnglish
Pages (from-to)811-824
Number of pages14
JournalJournal of Biomaterials Applications
Issue number8
Publication statusPublished - May 2011


  • bone formation.
  • fluoride-substituted apatite

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering


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