Effect of heat stress on serotonin-2A receptor-mediated intracellular calcium mobilization in rat C6 glioma cells

A. Kagaya, A. Okada, H. Jitsuiki, Y. Tawara, M. Inagaki, M. Takebayashi, T. Saeki, A. Nishida, Y. Nakata, S. Yamawaki

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


This study was conducted to investigate an effect of heat stress at 44 °C for 30min on intracellular Ca 2+ signaling system and on heat shock protein (HSP)-70 expression. 5-HT-induced Ca 2+ mobilization was reduced 1, 3 and 6hrs after heat stress, and recovered to the control level 12 and 24hrs after heat stress. One hr after heat stress, Ca 2+ rise was significantly decreased when the cells were stimulated by any concentration of 5-HT. Thrombin-induced Ca 2+ increase was also markedly reduced 1hr after heat stress. HSP-70 level was increased 6 and 9 hr after heat stress. In HSP synthesis inhibitor quercetin-treated cells, HSP-70 expression was not enhanced after heat stress, and Ca 2+ rise in response to 5-HT did not return to the control level. However, the Ca 2+ rise induced by 5-HT was not restored to the control level after stress in Ac-Asp-Glu-Val-Asp-H (DEVD)-exposed cells while DEVD had little effect on heat stress-induced synthesis of HSP-70. Dexamethasone did not alter the change in HSP-70 expression or Ca 2+ response after heat stress. These results indicate that heat stress attenuated 5-HT-induced Ca 2+ mobilization and that HSP-70 expression played an important role in recovery from Ca 2+ impairment, possibly via protease activity in C6 cells.

Original languageEnglish
Pages (from-to)919-929
Number of pages11
JournalJournal of Neural Transmission
Issue number8-9
Publication statusPublished - Oct 30 2000


  • 5-HT-2A receptors
  • Calcium
  • Heat shock protein
  • Quercetin
  • Serotonin

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Psychiatry and Mental health
  • Biological Psychiatry


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