Effect of streptococcal lyzate OK-432 on peripheral blood mononuclear cells in gastric cancer patients

Fumio Nakayama, Hiromi Iwagaki, Akira Gouchi, Akio Hizuta, Hiroshi Isozaki, Norihisa Takakura, Noriaki Tanaka

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2 Citations (Scopus)


OK-432, a killed preparation of Streptococcus pyogenes, as well as Bacillus Calmette-Guerin (BCG) and Corynebacterium parvum are all known biological response modifiers. To examine the immunomodulatory effects of OK- 432, natural killer cell activity and cytokine production by peripheral blood mononuclear cells (PBMCs) were assessed in 32 patients with gastric cancer. Skin tests for Streptococcus pyogenes A-3Su (Su-PS) and BCG were performed in all patients. Other nutritional and immunological parameters were also determined. OK-432-treated PBMCs showed a significant increase of cytotoxicity against K562 cells (p < 0.01). Increased levels of interferon- gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α) and interleukin-2 (IL- 2) were found in the supematants of cultures treated with OK-432 in 29 (90.6%), 20 (62.5%), and 8 (25.0%) out of 32 patients, respectively. Natural killer cell activity, IFN-γ production, and the Su-PS skin test were positively correlated (p < 0.01). In contrast, the BCG test and other markers were not correlated with natural killer cell activity and IFN-γ production. These results suggest that the Su-PS skin test could predict OK-432-induced natural killer cell activity and IFN-γ production in patients with gastric cancer, and was therefore useful to determine whether patients were responders to OK-432.

Original languageEnglish
Pages (from-to)199-215
Number of pages17
JournalJournal of Medicine
Issue number3-4
Publication statusPublished - 1998


  • Cytokines
  • Natural killer cell activity
  • Peripheral blood mononuclear cells
  • Streptococcal lyzate OK-432

ASJC Scopus subject areas

  • Medicine(all)


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