Effect of wortmannin on the suppression of thermotolerance in tsAF8 cells

Koichi Shibuya, Shoji Kawasaki, Masahiro Kuroda, Mitsuhiro Takemoto, Jun Ichi Asaumi, Yoshio Hiraki

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Phosphatidylinositol 3 kinases (PI-3 kinases) play a crucial role in the inositol phospholipid signaling pathway. The effect of wortmannin, an inhibitor of PI-3 kinases, on the suppression of thermotolerance development was investigated in the temperature-sensitive cell line tsAF8. When wortmannin was added immediately after the preheating, the thermotolerance was suppressed, with this suppression being dose-dependent from 0.01 to 0.1 μM, but not dose-dependent from 0.1 to 20 μM. Our results suggest that PI-3 kinases might play an important role in thermotolerance development in tsAF8 cells.

Original languageEnglish
Pages (from-to)81-84
Number of pages4
JournalOncology reports
Issue number1
Publication statusPublished - Jan 2002


  • PI-3 kinase
  • PKC
  • Thermotolerance
  • TsAF8
  • Wortmannin

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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