TY - JOUR
T1 - Effects of depletion of glutathione on abscisic acidand methyl jasmonate-induced stomatal closure in arabidopsis thaliana
AU - Akter, Nasima
AU - Sobahan, Muhammad Abdus
AU - Uraji, Misugi
AU - Ye, Wenxiu
AU - Hossain, Mohammad Anowar
AU - Mori, Izumi C.
AU - Nakamura, Yoshimasa
AU - Murata, Yoshiyuki
N1 - Funding Information:
This research was supported in part by a Grant-in-Aid for Young Scientists and Grants for Scientific Research on Priority Areas from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
PY - 2012
Y1 - 2012
N2 - Glutathione (GSH) is involved in abscisic acid (ABA)- and methyl jasmonate (MeJA)-induced stomatal closure in Arabidopsis thaliana. In this study, we examined the effects of GSH-decreasing chemicals, p-nitrobenzyl chloride (PNBC), iodomethane (IDM), and ethacrynic acid (EA), on ABA- and MeJA-induced stomatal closure in Arabidopsis. Treatments with PNBC, IDM, and EA decreased GSH contents in guard cells. Depletion of GSH by PNBC and IDM enhanced ABA- and MeJAinduced stomatal closure and inhibition of light-induced stomatal opening by ABA, whereas EA did not enhance either ABA- and MeJA-induced stomatal closure or inhibition of light-induced stomatal opening by ABA. Depletion of GSH did not significantly increase the production of the reactive oxygen species (ROS), cytosolic alkalization, or cytosolic Ca2+ oscillation induced by ABA and MeJA. These results indicate that depletion of GSH enhances ABA- and MeJA-induced stomatal closure without affecting ROS production, cytosolic alkalization, or cytosolic Ca2+ oscillation in guard cells of Arabidopsis.
AB - Glutathione (GSH) is involved in abscisic acid (ABA)- and methyl jasmonate (MeJA)-induced stomatal closure in Arabidopsis thaliana. In this study, we examined the effects of GSH-decreasing chemicals, p-nitrobenzyl chloride (PNBC), iodomethane (IDM), and ethacrynic acid (EA), on ABA- and MeJA-induced stomatal closure in Arabidopsis. Treatments with PNBC, IDM, and EA decreased GSH contents in guard cells. Depletion of GSH by PNBC and IDM enhanced ABA- and MeJAinduced stomatal closure and inhibition of light-induced stomatal opening by ABA, whereas EA did not enhance either ABA- and MeJA-induced stomatal closure or inhibition of light-induced stomatal opening by ABA. Depletion of GSH did not significantly increase the production of the reactive oxygen species (ROS), cytosolic alkalization, or cytosolic Ca2+ oscillation induced by ABA and MeJA. These results indicate that depletion of GSH enhances ABA- and MeJA-induced stomatal closure without affecting ROS production, cytosolic alkalization, or cytosolic Ca2+ oscillation in guard cells of Arabidopsis.
KW - Abscisic acid
KW - Cytosolic alkalization
KW - Glutathione
KW - Methyl jasmonate
KW - Reactive oxygen species
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U2 - 10.1271/bbb.120384
DO - 10.1271/bbb.120384
M3 - Article
C2 - 23132563
AN - SCOPUS:84870169809
SN - 0916-8451
VL - 76
SP - 2032
EP - 2037
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 11
ER -