TY - JOUR
T1 - Effects of human neutrophil peptide-1 on the expression of interstitial collagenase and type I collagen in human dermal fibroblasts
AU - Oono, Takashi
AU - Shirafuji, Yoshinori
AU - Huh, Wook Kang
AU - Akiyama, Hisanori
AU - Iwatsuki, Keiji
PY - 2002
Y1 - 2002
N2 - Human neutrophil peptide-1 (HNP-1), a defensin with antimicrobial properties, is also thought to promote wound healing. To elucidate the mechanism by which wound healing is facilitated by this factor, we investigated the effect of HNP-1 on the expression of interstitial collagenase (matrix metalloproteinase 1, MMP-1), collagen types I and III, and tissue inhibitor of metalloproteinase 1 (TIMP-1) by cultured fibroblasts by means of RT-PCR and ELISA. Our results showed that synthetic HNP-1 increased the expression of proα1(I) collagen mRNA and protein. In contrast, the expression of MMP-1 was decreased at both the mRNA and protein levels. Our observations suggest that HNP-1 may promote wound repair by enhancing extracellular matrix deposition and by controlling its degradation.
AB - Human neutrophil peptide-1 (HNP-1), a defensin with antimicrobial properties, is also thought to promote wound healing. To elucidate the mechanism by which wound healing is facilitated by this factor, we investigated the effect of HNP-1 on the expression of interstitial collagenase (matrix metalloproteinase 1, MMP-1), collagen types I and III, and tissue inhibitor of metalloproteinase 1 (TIMP-1) by cultured fibroblasts by means of RT-PCR and ELISA. Our results showed that synthetic HNP-1 increased the expression of proα1(I) collagen mRNA and protein. In contrast, the expression of MMP-1 was decreased at both the mRNA and protein levels. Our observations suggest that HNP-1 may promote wound repair by enhancing extracellular matrix deposition and by controlling its degradation.
KW - Collagen
KW - Collagenase
KW - Defensin
KW - Human neutrophil peptide
KW - Wound healing
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U2 - 10.1007/s00403-002-0310-6
DO - 10.1007/s00403-002-0310-6
M3 - Article
C2 - 12111349
AN - SCOPUS:0036019583
SN - 0340-3696
VL - 294
SP - 185
EP - 189
JO - Archives of Dermatological Research
JF - Archives of Dermatological Research
IS - 4
ER -