TY - JOUR
T1 - Effects of medium-chain fatty acids and their acylglycerols on the transport of penicillin V across Caco-2 cell monolayers
AU - Shima, Motohiro
AU - Yohdoh, Kaori
AU - Yamaguchi, Masayo
AU - Kimura, Yukitaka
AU - Adachi, Shuji
AU - Matsuno, Ryuichi
N1 - Funding Information:
Part of this study was supported by a Grant-in-Aid for Scientific Research on Priority Areas (No. 320) as "Analysis and Molecular Design of Functional Foods" by the Ministry of Education, Science, and Culture of Japan.
PY - 1997/1
Y1 - 1997/1
N2 - The transport-enhancing effects of medium-chain fatty acids (caproic, caprylic, and capric acids) and their acylglycerols (mono-, di-, and triacylglycerols) were investigated by using Caco-2 cell monolayers as a model of the human intestinal epithelium. Penicillin V was used as a model for a hydrophilic bioactive compound. Among the fatty acids and acylglycerols tested, 1,2-dicaproin, monocaprin, monocaprylin, and capric acid sodium salt effectively enhanced the transport rate, whereas other substances enhanced the rate only slightly or not at all. With each of these four substances, the rate of enhancement was proportional to the concentration at low concentrations, but leveled off at high concentrations. The transport-enhancing effects were well correlated with the reduction in surface tension and with a physico-chemical parameter, denoted by the surface energy-lowering coefficient, characterizing the surface activity of a substance.
AB - The transport-enhancing effects of medium-chain fatty acids (caproic, caprylic, and capric acids) and their acylglycerols (mono-, di-, and triacylglycerols) were investigated by using Caco-2 cell monolayers as a model of the human intestinal epithelium. Penicillin V was used as a model for a hydrophilic bioactive compound. Among the fatty acids and acylglycerols tested, 1,2-dicaproin, monocaprin, monocaprylin, and capric acid sodium salt effectively enhanced the transport rate, whereas other substances enhanced the rate only slightly or not at all. With each of these four substances, the rate of enhancement was proportional to the concentration at low concentrations, but leveled off at high concentrations. The transport-enhancing effects were well correlated with the reduction in surface tension and with a physico-chemical parameter, denoted by the surface energy-lowering coefficient, characterizing the surface activity of a substance.
KW - Caco-2
KW - Medium-chain fatty acids and acylglycerols
KW - Physico-chemical property
KW - Surface activity
KW - Transepithelial transport
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U2 - 10.1271/bbb.61.1150
DO - 10.1271/bbb.61.1150
M3 - Article
C2 - 9255979
AN - SCOPUS:0031183655
SN - 0916-8451
VL - 61
SP - 1150
EP - 1155
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 7
ER -