Effects of progesterone synthesized de novo in the developing purkinje cell on its dendritic growth and synaptogenesis

Hirotaka Sakamoto, Kazuyoshi Ukena, Kazuyoshi Tsutsui

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163 Citations (Scopus)


De novo steroidogenesis from cholesterol is a conserved property of vertebrate brains, and such steroids synthesized de novo in the brain are called neurosteroids. The identification of neurosteroidogenic cells is essential to the understanding of the physiological role of neurosteroids in the brain. We have demonstrated recently that neuronal neurosteroidogenesis occurs in the brain and indicated that the Purkinje cell actively synthesizes several neurosteroids de novo from cholesterol in vertebrates. Interestingly, in the rat, this neuron actively synthesizes progesterone de novo from cholesterol only during neonatal life, when cerebellar cortical formation occurs most markedly. Therefore, in this study, the possible organizing actions of progesterone during cerebellar development have been examined. In vitro studies using cerebellar slice cultures from newborn rats showed that progesterone promotes dose-dependent dendritic outgrowth of Purkinje cells but dose not affect their somata. This effect was blocked by the anti-progestin RU 486 [mifepristone; 17β-hydroxy-11β-(4-methylaminophenyl)-17α-(1-propynyl) estra-4,9-dien-3 one-6-7]. In vivo administration of progesterone to pups further revealed an increase in the density of Purkinje spine synapses electron microscopically. In contrast to progesterone, there was no significant effect of 3α,5α-tetrahydroprogesterone, a progesterone metabolite, on Purkinje cell development. Reverse transcription-PCR-Southern and immunocytochemical analyses showed that intranuclear progesterone receptors were expressed in Purkinje cells. These results suggest that progesterone promotes both dendritic outgrowth and synaptogenesis in Purkinje cells through intranuclear receptor-mediated mechanisms during cerebellar development. Such organizing actions may contribute to the formation of the cerebellar neuronal circuit.

Original languageEnglish
Pages (from-to)6221-6232
Number of pages12
JournalJournal of Neuroscience
Issue number16
Publication statusPublished - Aug 15 2001
Externally publishedYes


  • 3α,5α -tetrahydroprogesterone
  • Cerebellar cortical formation
  • Dendritic outgrowth
  • Development
  • Genomic action
  • Neurosteroids
  • Progesterone
  • Progesterone receptor
  • Purkinje cell
  • Synaptogenesis

ASJC Scopus subject areas

  • Neuroscience(all)


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