Effects of protein kinase A inhibitor (H-89) on VIP- and GRF-induced release and mRNA expression of prolactin and growth hormone in the chicken pituitary gland

Norio Kansaku; Kiyoshi Shimada; Noboru Saito; Hiroyoshi Hidaka

doi:10.1016/S0742-8413(97)00195-3

Effects of protein kinase A inhibitor (H-89) on VIP- and GRF-induced release and mRNA expression of prolactin and growth hormone in the chicken pituitary gland

Norio Kansaku, Kiyoshi Shimada, Noboru Saito, Hiroyoshi Hidaka

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Vasoactive intestinal polypeptide (VIP) and growth hormone releasing factor (GRF) stimulated an increase of cAMP accumulation with a concomitant release of PRL and GH, respectively. Release of PRL induced by VIP was partially suppressed by 5 and 25 μM of H-89, whereas VIP-induced gene expression of PRL was inhibited by all concentrations of H-89. Release and gene expression of GH induced by GRF was inhibited by H-89 in a dose dependent manner and completely blocked by 25 μM of H-89. These results indicate that VIP-induced PRL release and gene expression may be mediated, at least in a part, by cAMP-dependent protein kinase pachway, whereas GRF-induced GH release and gene expression may be mediated predominantly by cAMP dependent protein kinase.

Original language	English
Pages (from-to)	89-95
Number of pages	7
Journal	Comparative Biochemistry and Physiology - C Pharmacology Toxicology and Endocrinology
Volume	119
Issue number	1
DOIs	https://doi.org/10.1016/S0742-8413(97)00195-3
Publication status	Published - Jan 1998
Externally published	Yes

Keywords

Forskolin
GH
GRF
H-89
PKA
PRL
VIP
cAMP
mRMNA expression

ASJC Scopus subject areas

Immunology
Pharmacology

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10.1016/S0742-8413(97)00195-3

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Effects of protein kinase A inhibitor (H-89) on VIP- and GRF-induced release and mRNA expression of prolactin and growth hormone in the chicken pituitary gland. / Kansaku, Norio; Shimada, Kiyoshi; Saito, Noboru et al.
In: Comparative Biochemistry and Physiology - C Pharmacology Toxicology and Endocrinology, Vol. 119, No. 1, 01.1998, p. 89-95.

Research output: Contribution to journal › Article › peer-review

Kansaku, N, Shimada, K, Saito, N & Hidaka, H 1998, 'Effects of protein kinase A inhibitor (H-89) on VIP- and GRF-induced release and mRNA expression of prolactin and growth hormone in the chicken pituitary gland', Comparative Biochemistry and Physiology - C Pharmacology Toxicology and Endocrinology, vol. 119, no. 1, pp. 89-95. https://doi.org/10.1016/S0742-8413(97)00195-3

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title = "Effects of protein kinase A inhibitor (H-89) on VIP- and GRF-induced release and mRNA expression of prolactin and growth hormone in the chicken pituitary gland",

abstract = "Vasoactive intestinal polypeptide (VIP) and growth hormone releasing factor (GRF) stimulated an increase of cAMP accumulation with a concomitant release of PRL and GH, respectively. Release of PRL induced by VIP was partially suppressed by 5 and 25 μM of H-89, whereas VIP-induced gene expression of PRL was inhibited by all concentrations of H-89. Release and gene expression of GH induced by GRF was inhibited by H-89 in a dose dependent manner and completely blocked by 25 μM of H-89. These results indicate that VIP-induced PRL release and gene expression may be mediated, at least in a part, by cAMP-dependent protein kinase pachway, whereas GRF-induced GH release and gene expression may be mediated predominantly by cAMP dependent protein kinase.",

keywords = "Forskolin, GH, GRF, H-89, PKA, PRL, VIP, cAMP, mRMNA expression",

author = "Norio Kansaku and Kiyoshi Shimada and Noboru Saito and Hiroyoshi Hidaka",

note = "Funding Information: We thank Dr. A. F. Parlow (NIDDK and National Hormone and Pituitary Program) for providing the chicken PRL and GH RIA system, Dr. K. Nakashima (Mie University) for providing chicken PRL and GH cDNA, Drs. D. Zadworny (McGill University) and D. M. Olson (University of Alberta) for their help with reviewing the manuscript, and Dr. Wakabayashi (Gunma University) for providing anti-rabbit IgG of goat. We also appreciate the Radioisotope Research Center of Nagoya University for use of the Radioanalytic Image System BAS-2000. This research was supported by Grants from the Monbusho International Scientific Research Program (Joint Research, 07044190) to K. S. and Research Fellowships of the Japan Society for the Promotion of Science for young Scientists to N. K.",

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AU - Saito, Noboru

AU - Hidaka, Hiroyoshi

N1 - Funding Information: We thank Dr. A. F. Parlow (NIDDK and National Hormone and Pituitary Program) for providing the chicken PRL and GH RIA system, Dr. K. Nakashima (Mie University) for providing chicken PRL and GH cDNA, Drs. D. Zadworny (McGill University) and D. M. Olson (University of Alberta) for their help with reviewing the manuscript, and Dr. Wakabayashi (Gunma University) for providing anti-rabbit IgG of goat. We also appreciate the Radioisotope Research Center of Nagoya University for use of the Radioanalytic Image System BAS-2000. This research was supported by Grants from the Monbusho International Scientific Research Program (Joint Research, 07044190) to K. S. and Research Fellowships of the Japan Society for the Promotion of Science for young Scientists to N. K.

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N2 - Vasoactive intestinal polypeptide (VIP) and growth hormone releasing factor (GRF) stimulated an increase of cAMP accumulation with a concomitant release of PRL and GH, respectively. Release of PRL induced by VIP was partially suppressed by 5 and 25 μM of H-89, whereas VIP-induced gene expression of PRL was inhibited by all concentrations of H-89. Release and gene expression of GH induced by GRF was inhibited by H-89 in a dose dependent manner and completely blocked by 25 μM of H-89. These results indicate that VIP-induced PRL release and gene expression may be mediated, at least in a part, by cAMP-dependent protein kinase pachway, whereas GRF-induced GH release and gene expression may be mediated predominantly by cAMP dependent protein kinase.

AB - Vasoactive intestinal polypeptide (VIP) and growth hormone releasing factor (GRF) stimulated an increase of cAMP accumulation with a concomitant release of PRL and GH, respectively. Release of PRL induced by VIP was partially suppressed by 5 and 25 μM of H-89, whereas VIP-induced gene expression of PRL was inhibited by all concentrations of H-89. Release and gene expression of GH induced by GRF was inhibited by H-89 in a dose dependent manner and completely blocked by 25 μM of H-89. These results indicate that VIP-induced PRL release and gene expression may be mediated, at least in a part, by cAMP-dependent protein kinase pachway, whereas GRF-induced GH release and gene expression may be mediated predominantly by cAMP dependent protein kinase.

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Effects of protein kinase A inhibitor (H-89) on VIP- and GRF-induced release and mRNA expression of prolactin and growth hormone in the chicken pituitary gland

Abstract

Keywords

ASJC Scopus subject areas

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