Effects of quercetin on mushroom tyrosinase and B16-F10 melanoma cells

Isao Kubo, Teruhiko Nitoda, Ken Ichi Nihei

Research output: Contribution to journalArticlepeer-review

58 Citations (Scopus)


In searching for tyrosinase inhibitors from plants using L-3,4-dihydroxyphenylalanine (L-DOPA) as a substrate, quercetin was found to be partially oxidized to the corresponding o-quinone under catalysis by mushroom tyrosinase (EC Simultaneously, L-DOPA was also oxidized to dopaquinone and both o-quinones were further oxidized, respectively. The remaining quercetin partially formed adducts with dopaquinone through a Michael type addition. In general, flavonols form adducts with dopaquinone as long as their 3-hydroxyl group is free. Quercetin enhanced melanin production per cell in cultured murine B16-F10 melanoma cells, but this effect may be due in part to melanocytotoxicity. The concentration leading to 50% viable cells lost was established as 20 μM and almost complete lethality was observed at 80 μM.

Original languageEnglish
Pages (from-to)1045-1056
Number of pages12
Issue number5
Publication statusPublished - May 2007
Externally publishedYes


  • Adducts
  • Cytotoxicity
  • Murine B16-F10 melanoma cells
  • Mushroom tyrosinase
  • Quercetin

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry


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