Effects of tensile and compressive strains on response of a chondrocytic cell line embedded in type I collagen gel

Yuji Hirano, Naoki Ishiguro, Masahiro Sokabe, Masaharu Takigawa, Keiji Naruse

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)


Tensile and compressive strains are commonly used in mechanobiological models. Here we report on the development of a novel three-dimensional cell-culture method, which allows both tensile and compressive loads to be applied. Preliminary results were obtained using HCS2/8 chondrocytic cells embedded in type I collagen gel. This construct was subjected to either 16% tension or 14% compression. Confocal laser scanning microscopy showed that both tension and compression caused significant cell deformation. The collagen gel-embedded HCS2/8 cells were subjected to static tension, dynamic tension, static compression or dynamic compression for 24 h. Dynamic compression led to significantly decreased 5-bromo-2′-deoxyuridine incorporation compared with the control group. PCR analysis revealed upregulation of type II collagen caused by dynamic tension, upregulation of aggrecan caused by static compression, and downregulation of type II collagen and aggrecan caused by dynamic compression. Nitric oxide production was significantly increased by static tension and static compression compared with the control group. Our experimental system effectively applied several types of strain to HCS2/8 cells embedded in collagen gel. Our results suggest that the mode of mechanical strain affects the response of HCS2/8 cells.

Original languageEnglish
Pages (from-to)245-252
Number of pages8
JournalJournal of biotechnology
Issue number2
Publication statusPublished - Jan 20 2008


  • Chondrocytes
  • Compression
  • Mechanical strain
  • Tension
  • Three-dimensional culture

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


Dive into the research topics of 'Effects of tensile and compressive strains on response of a chondrocytic cell line embedded in type I collagen gel'. Together they form a unique fingerprint.

Cite this