Efficient Cre/loxP site-specific recombination in a HepG2 human liver cell line

Naoya Kobayashi, Hirofumi Noguchi, Karen A. Westerman, Toshihisa Matsumura, Takamasa Watanabe, Toshinori Totsugawa, Toshiyoshi Fujiwara, Philippe Leboulch, Noriaki Tanaka

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


A worldwide shortage of donor livers is a limiting factor of the clinical application of hepatocyte transplantation (HTX). To resolve this issue, we focused on a reversible immortalization system that allows temporary expansion of primary hepatocyte populations by transfer of an oncogene that can be subsequently excised. As a preliminary test toward this goal, we examined the efficacy of Cre/loxP site-specific recombination in a transformed human liver cell line, HepG2. The present study utilized retroviral transfer of a prototypical immortalizing gene, simian virus 40 large T antigen (SV40Tag), flanked by a pair of loxP recombination targets and adenovirus-mediated Cre/loxP recombination. Here we report that complete elimination of the retroviral transferred oncogene was achieved by site-specific recombination using a replication-deficient recombinant adenovirus vector producing Cre recombinase (Ad-Cre).

Original languageEnglish
Pages (from-to)737-742
Number of pages6
JournalCell Transplantation
Issue number5
Publication statusPublished - Jan 1 2000


  • Cre/loxP site-specific recombination
  • HepG2 cells
  • Reversible immortalization

ASJC Scopus subject areas

  • Biomedical Engineering
  • Cell Biology
  • Transplantation


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