Efficient enhancement of priming effect by intermittent treatment with interferon

Fusao Ikeda, Hiroyuki Shimomura, Hiroshi Nakagawa, Yoshiaki Iwasaki, Masanobu Miyake, Hideyuki Tsuji, Shin ichi Fukioka, Mamoru Itoh, Akira Takahashi, Takao Tsuji

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


To improve the efficacy of interferon (IFN) therapy for chronic hepatitis C, we proposed a therapy with twice-a-day injection of IFNβ as the induction. To assess its biological enhancement, we compared antiviral activities in vitro using intermittent treatment schedules simulating the clinical condition. FL cells were treated with IFNβ twice in 12 h interval (Single treatment, 1000 and 0 IU/ml; Double treatment, 500 IU/ml each) and challenged with Sindbis virus. Antiviral activities were determined with 50% cytopathic effect. Activities and mRNA expressions of 2'5'oligoadenylate synthetase (2'5'AS) were also examined. Single treatment showed its peak activity at 9 h, while Double treatment was at 3 h after the second treatment. Double treatment had a significantly higher peak activity. The up-regulated activities of 2'5'AS lasted much longer with Double treatment. The present findings demonstrated Double treatment could induce efficient biological enhancement, which is thought based on the priming effect. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)599-603
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number3
Publication statusPublished - Nov 30 2000


  • Antiviral effect
  • Chronic hepatitis C
  • Interferon
  • Intermittent treatment
  • Priming effect

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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