TY - JOUR
T1 - EGFR and ERK activation resists flavonoid quercetin-induced anticancer activities in human cervical cancer cells in vitro
AU - Chen, Xin
AU - Xu, Pengli
AU - Zhang, Huijun
AU - Su, Xiaosan
AU - Guo, Lihua
AU - Zhou, Xuhong
AU - Wang, Junliang
AU - Huang, Peng
AU - Zhang, Qingzhi
AU - Sun, Ruifen
N1 - Funding Information:
This study was supported in part by the National Natural Science Foundation of China (grant nos. 81560429, 81602545 and 81760519) and the Yunnan Natural Science Foundation of China [grant nos. 2017FF116 (‑023) and 2019FF002 (‑011)]
Funding Information:
This study was supported in part by the National Natural Science Foundation of China (grant nos. 81560429, 81602545 and 81760519) and the Yunnan Natural Science Foundation of China [grant nos. 2017FF116 (-023) and 2019FF002 (-011)]
Publisher Copyright:
© 2021 Spandidos Publications. All rights reserved.
PY - 2021/11
Y1 - 2021/11
N2 - In the present study, due to the complex and numerous targets of Sarcandrae Herb (also known as Zhong Jie Feng), network pharmacology was performed to analyze its therapeutic effect on 2 cervical cancer cell lines, which could assist with the development of novel therapies. The results suggested that the natural flavonoid quercetin (Que), the effective antitumor ingre- dient in SH, which is widely present in a variety of plants, may depend on the target, EGFR. Previous studies have shown that EGFR serves a crucial role in the occurrence and development of cervical cancer, but its downstream molecules and regula- tory mechanisms remain unknown. The anti-cervical cancer cell properties of Que, which are present in ubiquitous plants, were examined in vitro to identify the association between Que and its underlying pathway using MTT assays, flow cytometry, western blot analysis and Transwell assays. It was found that Que reduced cervical cancer cell viability, promoted G2/M phase cell cycle arrest and cell apoptosis, as well as inhibited cell migration and invasion. The Tyr1068 phosphorylation site of EGFR and the corresponding ERK target were also examined and the 2 kinases were markedly activated by Que. Furthermore, the EGFR inhibitor, afatinib and the ERK inhibitor, U0126 blocked the increase of EGFR and ERK phos- phorylation, and resulted in a notable enhancement of apoptosis and cell cycle arrest. Therefore, to the best of our knowledge, the current results provided the first evidence that EGFR and ERK activation induced by Que could resist Que-induced anticancer activities. On this basis, the present study determined the role of EGFR and the underlying signaling pathways involved in the anti-cervical cancer malignant behavior induced by Que and identified the negative regulatory association.
AB - In the present study, due to the complex and numerous targets of Sarcandrae Herb (also known as Zhong Jie Feng), network pharmacology was performed to analyze its therapeutic effect on 2 cervical cancer cell lines, which could assist with the development of novel therapies. The results suggested that the natural flavonoid quercetin (Que), the effective antitumor ingre- dient in SH, which is widely present in a variety of plants, may depend on the target, EGFR. Previous studies have shown that EGFR serves a crucial role in the occurrence and development of cervical cancer, but its downstream molecules and regula- tory mechanisms remain unknown. The anti-cervical cancer cell properties of Que, which are present in ubiquitous plants, were examined in vitro to identify the association between Que and its underlying pathway using MTT assays, flow cytometry, western blot analysis and Transwell assays. It was found that Que reduced cervical cancer cell viability, promoted G2/M phase cell cycle arrest and cell apoptosis, as well as inhibited cell migration and invasion. The Tyr1068 phosphorylation site of EGFR and the corresponding ERK target were also examined and the 2 kinases were markedly activated by Que. Furthermore, the EGFR inhibitor, afatinib and the ERK inhibitor, U0126 blocked the increase of EGFR and ERK phos- phorylation, and resulted in a notable enhancement of apoptosis and cell cycle arrest. Therefore, to the best of our knowledge, the current results provided the first evidence that EGFR and ERK activation induced by Que could resist Que-induced anticancer activities. On this basis, the present study determined the role of EGFR and the underlying signaling pathways involved in the anti-cervical cancer malignant behavior induced by Que and identified the negative regulatory association.
KW - Cervical cancer
KW - EGFR
KW - ERK
KW - Negative regulatory association
KW - Quercetin
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U2 - 10.3892/ol.2021.13015
DO - 10.3892/ol.2021.13015
M3 - Article
AN - SCOPUS:85114252864
SN - 1792-1074
VL - 22
JO - Oncology Letters
JF - Oncology Letters
IS - 5
M1 - 754
ER -