Elevation of interleukin-6 in inflammatory bowel disease is macrophage- and epithelial cell-dependent

Kazuo Kusugami; Atsushi Fukatsu; Mitsune Tanimoto; Masataka Shinoda; Jun Ichi Haruta; Atsuo Kuroiwa; Kenji Ina; Kazuhiro Kanayama; Takafumi Ando; Toshihiro Matsuura; Takeo Yamaguchi; Kimitomo Morise; Mayumi Ieda; Hitoshi Iokawa; Akira Ishihara; Susumu Sarai

doi:10.1007/BF02064182

Elevation of interleukin-6 in inflammatory bowel disease is macrophage- and epithelial cell-dependent

Kazuo Kusugami, Atsushi Fukatsu, Mitsune Tanimoto, Masataka Shinoda, Jun Ichi Haruta, Atsuo Kuroiwa, Kenji Ina, Kazuhiro Kanayama, Takafumi Ando, Toshihiro Matsuura, Takeo Yamaguchi, Kimitomo Morise, Mayumi Ieda, Hitoshi Iokawa, Akira Ishihara, Susumu Sarai

Graduate School of Medicine Dentistry and Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

142 Citations (Scopus)

Abstract

Local interleukin-6 (IL-6) activity was studied using colonic mucosal tissues in inflammatory bowel disease (IBD) and inflammatory control patients. Active IBD specimens exhibited significantly higher IL-6 activity than control specimens in both cultures of isolated lamina propria mononuclear cells (LPMC) and mucosal tissues with an increased number of IL-6-producing cells. However, the activity in inactive IBD or inflammatory controls did not differ from controls. Northern blot analysis demonstrated IL-6 messenger RNA in LPMC and colonic epithelial cells isolated from active IBD specimens but not in control cells. Furthermore, immunofluorescent microscopic study of active IBD specimens showed more conspicuous staining of IL-6 in infiltrating LPMC (mostly CD68⁺ cells) and colonic epithelial cells. These results suggest that elevation of local IL-6 activity may be a characteristic feature of active IBD and both macrophages and colonic epithelial cells are the major cell types responsible for this phenomenon.

Original language	English
Pages (from-to)	949-959
Number of pages	11
Journal	Digestive Diseases and Sciences
Volume	40
Issue number	5
DOIs	https://doi.org/10.1007/BF02064182
Publication status	Published - May 1995

Keywords

colonic epithelial cells
inflammatory bowel disease
interleukin-6
macrophages

ASJC Scopus subject areas

Physiology
Gastroenterology

Access to Document

10.1007/BF02064182

Cite this

Kusugami, K., Fukatsu, A., Tanimoto, M., Shinoda, M., Haruta, J. I., Kuroiwa, A., Ina, K., Kanayama, K., Ando, T., Matsuura, T., Yamaguchi, T., Morise, K., Ieda, M., Iokawa, H., Ishihara, A., & Sarai, S. (1995). Elevation of interleukin-6 in inflammatory bowel disease is macrophage- and epithelial cell-dependent. Digestive Diseases and Sciences, 40(5), 949-959. https://doi.org/10.1007/BF02064182

Kusugami, K, Fukatsu, A, Tanimoto, M, Shinoda, M, Haruta, JI, Kuroiwa, A, Ina, K, Kanayama, K, Ando, T, Matsuura, T, Yamaguchi, T, Morise, K, Ieda, M, Iokawa, H, Ishihara, A & Sarai, S 1995, 'Elevation of interleukin-6 in inflammatory bowel disease is macrophage- and epithelial cell-dependent', Digestive Diseases and Sciences, vol. 40, no. 5, pp. 949-959. https://doi.org/10.1007/BF02064182

@article{9dd2717cd3be497ebf6ad2f8cea7dcbb,

title = "Elevation of interleukin-6 in inflammatory bowel disease is macrophage- and epithelial cell-dependent",

abstract = "Local interleukin-6 (IL-6) activity was studied using colonic mucosal tissues in inflammatory bowel disease (IBD) and inflammatory control patients. Active IBD specimens exhibited significantly higher IL-6 activity than control specimens in both cultures of isolated lamina propria mononuclear cells (LPMC) and mucosal tissues with an increased number of IL-6-producing cells. However, the activity in inactive IBD or inflammatory controls did not differ from controls. Northern blot analysis demonstrated IL-6 messenger RNA in LPMC and colonic epithelial cells isolated from active IBD specimens but not in control cells. Furthermore, immunofluorescent microscopic study of active IBD specimens showed more conspicuous staining of IL-6 in infiltrating LPMC (mostly CD68+ cells) and colonic epithelial cells. These results suggest that elevation of local IL-6 activity may be a characteristic feature of active IBD and both macrophages and colonic epithelial cells are the major cell types responsible for this phenomenon.",

keywords = "colonic epithelial cells, inflammatory bowel disease, interleukin-6, macrophages",

author = "Kazuo Kusugami and Atsushi Fukatsu and Mitsune Tanimoto and Masataka Shinoda and Haruta, {Jun Ichi} and Atsuo Kuroiwa and Kenji Ina and Kazuhiro Kanayama and Takafumi Ando and Toshihiro Matsuura and Takeo Yamaguchi and Kimitomo Morise and Mayumi Ieda and Hitoshi Iokawa and Akira Ishihara and Susumu Sarai",

year = "1995",

month = may,

doi = "10.1007/BF02064182",

language = "English",

volume = "40",

pages = "949--959",

journal = "Digestive Diseases and Sciences",

issn = "0163-2116",

publisher = "Springer New York",

number = "5",

}

TY - JOUR

T1 - Elevation of interleukin-6 in inflammatory bowel disease is macrophage- and epithelial cell-dependent

AU - Kusugami, Kazuo

AU - Fukatsu, Atsushi

AU - Tanimoto, Mitsune

AU - Shinoda, Masataka

AU - Haruta, Jun Ichi

AU - Kuroiwa, Atsuo

AU - Ina, Kenji

AU - Kanayama, Kazuhiro

AU - Ando, Takafumi

AU - Matsuura, Toshihiro

AU - Yamaguchi, Takeo

AU - Morise, Kimitomo

AU - Ieda, Mayumi

AU - Iokawa, Hitoshi

AU - Ishihara, Akira

AU - Sarai, Susumu

PY - 1995/5

Y1 - 1995/5

N2 - Local interleukin-6 (IL-6) activity was studied using colonic mucosal tissues in inflammatory bowel disease (IBD) and inflammatory control patients. Active IBD specimens exhibited significantly higher IL-6 activity than control specimens in both cultures of isolated lamina propria mononuclear cells (LPMC) and mucosal tissues with an increased number of IL-6-producing cells. However, the activity in inactive IBD or inflammatory controls did not differ from controls. Northern blot analysis demonstrated IL-6 messenger RNA in LPMC and colonic epithelial cells isolated from active IBD specimens but not in control cells. Furthermore, immunofluorescent microscopic study of active IBD specimens showed more conspicuous staining of IL-6 in infiltrating LPMC (mostly CD68+ cells) and colonic epithelial cells. These results suggest that elevation of local IL-6 activity may be a characteristic feature of active IBD and both macrophages and colonic epithelial cells are the major cell types responsible for this phenomenon.

AB - Local interleukin-6 (IL-6) activity was studied using colonic mucosal tissues in inflammatory bowel disease (IBD) and inflammatory control patients. Active IBD specimens exhibited significantly higher IL-6 activity than control specimens in both cultures of isolated lamina propria mononuclear cells (LPMC) and mucosal tissues with an increased number of IL-6-producing cells. However, the activity in inactive IBD or inflammatory controls did not differ from controls. Northern blot analysis demonstrated IL-6 messenger RNA in LPMC and colonic epithelial cells isolated from active IBD specimens but not in control cells. Furthermore, immunofluorescent microscopic study of active IBD specimens showed more conspicuous staining of IL-6 in infiltrating LPMC (mostly CD68+ cells) and colonic epithelial cells. These results suggest that elevation of local IL-6 activity may be a characteristic feature of active IBD and both macrophages and colonic epithelial cells are the major cell types responsible for this phenomenon.

KW - colonic epithelial cells

KW - inflammatory bowel disease

KW - interleukin-6

KW - macrophages

UR - http://www.scopus.com/inward/record.url?scp=0029017776&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029017776&partnerID=8YFLogxK

U2 - 10.1007/BF02064182

DO - 10.1007/BF02064182

M3 - Article

C2 - 7729284

AN - SCOPUS:0029017776

SN - 0163-2116

VL - 40

SP - 949

EP - 959

JO - Digestive Diseases and Sciences

JF - Digestive Diseases and Sciences

IS - 5

ER -

Elevation of interleukin-6 in inflammatory bowel disease is macrophage- and epithelial cell-dependent

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this