Elicitin-responsive lectin-like receptor kinase genes in BY-2 cells

Michiko Sasabe; Kana Naito; Hiroko Suenaga; Takako Ikeda; Kazuhiro Toyoda; Yoshishige Inagaki; Tomonori Shiraishi; Yuki Ichinose

doi:10.1080/10425170601060905

Elicitin-responsive lectin-like receptor kinase genes in BY-2 cells

Michiko Sasabe, Kana Naito, Hiroko Suenaga, Takako Ikeda, Kazuhiro Toyoda, Yoshishige Inagaki, Tomonori Shiraishi, Yuki Ichinose

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

The inhibition of elicitor-induced plant defense responses by the protein kinase inhibitors K252a and staurosporine indicates that defense responses require protein phosphorylation. We isolated a cDNA clone encoding Nicotiana tabacum lectin-like receptor protein kinase 1 (NtlecRK1), an elicitor-responsive gene; in tobacco bright yellow (BY-2) cells by a differential display method. NtlecRK forms a gene family with at least three members in tobacco. All three NtlecRK genes potentially encode the N-terminal legume lectin domain, transmembrane domain and C-terminal Ser/Thr-type protein kinase domain. Green fluorescent protein (GFP) fusion showed that the NtlecRK1 protein was located on the plasma membrane. In addition, NtlecRK1 and 3 were responsive to INF1 elicitin and the bacterial elicitor harpin. These results indicate that NtlecRKs are membrane-located protein kinases that are induced during defense responses in BY-2 cells.

Original language	English
Pages (from-to)	152-159
Number of pages	8
Journal	DNA Sequence - Journal of DNA Sequencing and Mapping
Volume	18
Issue number	2
DOIs	https://doi.org/10.1080/10425170601060905
Publication status	Published - 2007

Keywords

Defense response
Elicitin
Lectin
Receptor-like kinase
Tobacco BY-2

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Genetics
Endocrinology

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10.1080/10425170601060905

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title = "Elicitin-responsive lectin-like receptor kinase genes in BY-2 cells",

abstract = "The inhibition of elicitor-induced plant defense responses by the protein kinase inhibitors K252a and staurosporine indicates that defense responses require protein phosphorylation. We isolated a cDNA clone encoding Nicotiana tabacum lectin-like receptor protein kinase 1 (NtlecRK1), an elicitor-responsive gene; in tobacco bright yellow (BY-2) cells by a differential display method. NtlecRK forms a gene family with at least three members in tobacco. All three NtlecRK genes potentially encode the N-terminal legume lectin domain, transmembrane domain and C-terminal Ser/Thr-type protein kinase domain. Green fluorescent protein (GFP) fusion showed that the NtlecRK1 protein was located on the plasma membrane. In addition, NtlecRK1 and 3 were responsive to INF1 elicitin and the bacterial elicitor harpin. These results indicate that NtlecRKs are membrane-located protein kinases that are induced during defense responses in BY-2 cells.",

keywords = "Defense response, Elicitin, Lectin, Receptor-like kinase, Tobacco BY-2",

author = "Michiko Sasabe and Kana Naito and Hiroko Suenaga and Takako Ikeda and Kazuhiro Toyoda and Yoshishige Inagaki and Tomonori Shiraishi and Yuki Ichinose",

note = "Funding Information: We thank Prof. Sophien Kamoun, Ohio State University, for the gift of the inf1 gene, Dr Yasuo Niwa, Shizuoka, University for providing the CaMV35S Χ-sGFP(S65T)-nos30 plasmid, and Dr Fumiko Taguchi in our laboratory for the preparation of harpinPsg. This work was supported by a Research Fellowship of the Japan Society for the Promotion of Science for Young Scientists to MS and also by Grants-in-Aid for Scientific Research (S) (No. 15108001) and Priority Areas (No. 12052215) from the Ministry of Education, Culture, Sports, Science and Technology of Japan and the Okayama University COE program “Establishment of Plant Health Science”.",

year = "2007",

doi = "10.1080/10425170601060905",

language = "English",

volume = "18",

pages = "152--159",

journal = "DNA Sequence - Journal of DNA Sequencing and Mapping",

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T1 - Elicitin-responsive lectin-like receptor kinase genes in BY-2 cells

AU - Sasabe, Michiko

AU - Naito, Kana

AU - Suenaga, Hiroko

AU - Ikeda, Takako

AU - Toyoda, Kazuhiro

AU - Inagaki, Yoshishige

AU - Shiraishi, Tomonori

AU - Ichinose, Yuki

N1 - Funding Information: We thank Prof. Sophien Kamoun, Ohio State University, for the gift of the inf1 gene, Dr Yasuo Niwa, Shizuoka, University for providing the CaMV35S Χ-sGFP(S65T)-nos30 plasmid, and Dr Fumiko Taguchi in our laboratory for the preparation of harpinPsg. This work was supported by a Research Fellowship of the Japan Society for the Promotion of Science for Young Scientists to MS and also by Grants-in-Aid for Scientific Research (S) (No. 15108001) and Priority Areas (No. 12052215) from the Ministry of Education, Culture, Sports, Science and Technology of Japan and the Okayama University COE program “Establishment of Plant Health Science”.

PY - 2007

Y1 - 2007

N2 - The inhibition of elicitor-induced plant defense responses by the protein kinase inhibitors K252a and staurosporine indicates that defense responses require protein phosphorylation. We isolated a cDNA clone encoding Nicotiana tabacum lectin-like receptor protein kinase 1 (NtlecRK1), an elicitor-responsive gene; in tobacco bright yellow (BY-2) cells by a differential display method. NtlecRK forms a gene family with at least three members in tobacco. All three NtlecRK genes potentially encode the N-terminal legume lectin domain, transmembrane domain and C-terminal Ser/Thr-type protein kinase domain. Green fluorescent protein (GFP) fusion showed that the NtlecRK1 protein was located on the plasma membrane. In addition, NtlecRK1 and 3 were responsive to INF1 elicitin and the bacterial elicitor harpin. These results indicate that NtlecRKs are membrane-located protein kinases that are induced during defense responses in BY-2 cells.

AB - The inhibition of elicitor-induced plant defense responses by the protein kinase inhibitors K252a and staurosporine indicates that defense responses require protein phosphorylation. We isolated a cDNA clone encoding Nicotiana tabacum lectin-like receptor protein kinase 1 (NtlecRK1), an elicitor-responsive gene; in tobacco bright yellow (BY-2) cells by a differential display method. NtlecRK forms a gene family with at least three members in tobacco. All three NtlecRK genes potentially encode the N-terminal legume lectin domain, transmembrane domain and C-terminal Ser/Thr-type protein kinase domain. Green fluorescent protein (GFP) fusion showed that the NtlecRK1 protein was located on the plasma membrane. In addition, NtlecRK1 and 3 were responsive to INF1 elicitin and the bacterial elicitor harpin. These results indicate that NtlecRKs are membrane-located protein kinases that are induced during defense responses in BY-2 cells.

KW - Defense response

KW - Elicitin

KW - Lectin

KW - Receptor-like kinase

KW - Tobacco BY-2

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JO - DNA Sequence - Journal of DNA Sequencing and Mapping

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ER -

Elicitin-responsive lectin-like receptor kinase genes in BY-2 cells

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Keywords

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