Elucidating the site of action of oxalate in photosynthetic electron transport chain in spinach thylakoid membranes

Anjana Jajoo; Archna Sahay; Pooja Singh; Sonal Mathur; Sergei K. Zharmukhamedov; Vyacheslav V. Klimov; Suleyman I. Allakhverdiev; Sudhakar Bharti

doi:10.1007/s11120-008-9314-1

Elucidating the site of action of oxalate in photosynthetic electron transport chain in spinach thylakoid membranes

Anjana Jajoo, Archna Sahay, Pooja Singh, Sonal Mathur, Sergei K. Zharmukhamedov, Vyacheslav V. Klimov, Suleyman I. Allakhverdiev, Sudhakar Bharti

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The effects of oxalate on PS II and PS I photochemistry were studied. The results suggested that in chloride-deficient thylakoid membranes, oxalate inhibited activity of PS II as well as PS I. To our knowledge, this is the only anion so far known which inhibits both the photosystems. Measurements of fluorescence induction kinetics, Y _Z ^• decay, and S₂ state multiline EPR signal suggested that oxalate inhibited PS II at the donor side most likely on the oxygen evolving complex. Measurements of re-reduction of P700⁺ signal in isolated PS I particles in oxalate-treated samples suggested a binding site of oxalate on the donor, as well as the acceptor side of PS I.

Original language	English
Pages (from-to)	177-184
Number of pages	8
Journal	Photosynthesis research
Volume	97
Issue number	2
DOIs	https://doi.org/10.1007/s11120-008-9314-1
Publication status	Published - Aug 2008

Keywords

EPR
Electron transport chain
Fluorescence
Oxalate anion
Photosystem I
Photosystem II

ASJC Scopus subject areas

Biochemistry
Plant Science
Cell Biology

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10.1007/s11120-008-9314-1

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title = "Elucidating the site of action of oxalate in photosynthetic electron transport chain in spinach thylakoid membranes",

abstract = "The effects of oxalate on PS II and PS I photochemistry were studied. The results suggested that in chloride-deficient thylakoid membranes, oxalate inhibited activity of PS II as well as PS I. To our knowledge, this is the only anion so far known which inhibits both the photosystems. Measurements of fluorescence induction kinetics, Y Z • decay, and S2 state multiline EPR signal suggested that oxalate inhibited PS II at the donor side most likely on the oxygen evolving complex. Measurements of re-reduction of P700+ signal in isolated PS I particles in oxalate-treated samples suggested a binding site of oxalate on the donor, as well as the acceptor side of PS I.",

keywords = "EPR, Electron transport chain, Fluorescence, Oxalate anion, Photosystem I, Photosystem II",

author = "Anjana Jajoo and Archna Sahay and Pooja Singh and Sonal Mathur and Zharmukhamedov, {Sergei K.} and Klimov, {Vyacheslav V.} and Allakhverdiev, {Suleyman I.} and Sudhakar Bharti",

note = "Funding Information: Acknowledgment This work was supported by the Indo-Russian Joint project INT/ILTP/B-6.27 and by grants from the Russian Foundation for Basic Research.",

year = "2008",

month = aug,

doi = "10.1007/s11120-008-9314-1",

language = "English",

volume = "97",

pages = "177--184",

journal = "Photosynthesis Research",

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T1 - Elucidating the site of action of oxalate in photosynthetic electron transport chain in spinach thylakoid membranes

AU - Jajoo, Anjana

AU - Sahay, Archna

AU - Singh, Pooja

AU - Mathur, Sonal

AU - Zharmukhamedov, Sergei K.

AU - Klimov, Vyacheslav V.

AU - Allakhverdiev, Suleyman I.

AU - Bharti, Sudhakar

N1 - Funding Information: Acknowledgment This work was supported by the Indo-Russian Joint project INT/ILTP/B-6.27 and by grants from the Russian Foundation for Basic Research.

PY - 2008/8

Y1 - 2008/8

N2 - The effects of oxalate on PS II and PS I photochemistry were studied. The results suggested that in chloride-deficient thylakoid membranes, oxalate inhibited activity of PS II as well as PS I. To our knowledge, this is the only anion so far known which inhibits both the photosystems. Measurements of fluorescence induction kinetics, Y Z • decay, and S2 state multiline EPR signal suggested that oxalate inhibited PS II at the donor side most likely on the oxygen evolving complex. Measurements of re-reduction of P700+ signal in isolated PS I particles in oxalate-treated samples suggested a binding site of oxalate on the donor, as well as the acceptor side of PS I.

AB - The effects of oxalate on PS II and PS I photochemistry were studied. The results suggested that in chloride-deficient thylakoid membranes, oxalate inhibited activity of PS II as well as PS I. To our knowledge, this is the only anion so far known which inhibits both the photosystems. Measurements of fluorescence induction kinetics, Y Z • decay, and S2 state multiline EPR signal suggested that oxalate inhibited PS II at the donor side most likely on the oxygen evolving complex. Measurements of re-reduction of P700+ signal in isolated PS I particles in oxalate-treated samples suggested a binding site of oxalate on the donor, as well as the acceptor side of PS I.

KW - EPR

KW - Electron transport chain

KW - Fluorescence

KW - Oxalate anion

KW - Photosystem I

KW - Photosystem II

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SN - 0166-8595

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EP - 184

JO - Photosynthesis Research

JF - Photosynthesis Research

IS - 2

ER -

Elucidating the site of action of oxalate in photosynthetic electron transport chain in spinach thylakoid membranes

Abstract

Keywords

ASJC Scopus subject areas

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