@article{d5e0f6d2d7a240d1a5e8988136ca41ac,
title = "Engineering rice with lower grain arsenic",
abstract = "Arsenic (As) is a poisonous element that causes severe skin lesions and cancer in humans. Rice (Oryza sativa L.) is a major dietary source of As in humans who consume this cereal as a staple food. We hypothesized that increasing As vacuolar sequestration would inhibit its translocation into the grain and reduce the amount of As entering the food chain. We developed transgenic rice plants expressing two different vacuolar As sequestration genes, ScYCF1 and OsABCC1, under the control of the RCc3 promoter in the root cortical and internode phloem cells, along with a bacterial γ-glutamylcysteine synthetase driven by the maize UBI promoter. The transgenic rice plants exhibited reduced root-to-shoot and internode-to-grain As translocation, resulting in a 70% reduction in As accumulation in the brown rice without jeopardizing agronomic traits. This technology could be used to reduce As intake, particularly in populations of South East Asia suffering from As toxicity and thereby improve human health.",
keywords = "ABC transporter, arsenic, rice, vacuolar sequestration",
author = "Fenglin Deng and Naoki Yamaji and Ma, {Jian Feng} and Lee, {Sang Kyu} and Jeon, {Jong Seong} and Enrico Martinoia and Youngsook Lee and Song, {Won Yong}",
note = "Funding Information: This work was supported by grants from the BK21 PLUS program funded by the Ministry of Education, Korea (10Z20130012243), the Next-Generation BioGreen 21 program from Rural Development Administration, Korea (PJ011926 to W-Y.S.), NRF grants funded by the Korean government (MSIP) (NRF-2016R1A2B4012478 to W-Y.S., NRF-2015R1A2A1A01004294 to Y.L), and a grant for Specially Promoted Research (JSPS KAKENHI Grant Number 16H06296 to J.F.M.). We thank the POCEB at Pohang Techno Park for the use of the ICP-MS (NexIon300x, Perkin Elmer) facility. The authors declare competing financial interests: ?Composition for reducing arsenic accumulation? is the subject of a Korea patent (10-2017-0060054 on May 15, 2017) applied by W-Y.S, Y.L and F.D. Funding Information: This work was supported by grants from the BK21 PLUS program funded by the Ministry of Education, Korea (10Z20130012243), the Next-Generation BioGreen 21 program from Rural Development Administration, Korea (PJ011926 to W-Y.S.), NRF grants funded by the Korean government (MSIP) (NRF-2016R1A2B4012478 to W-Y.S., NRF-2015R1A2A1A01004294 to Y.L), and a grant for Specially Promoted Research (JSPS KAKENHI Grant Number 16H06296 to J.F.M.). We thank the POCEB at Pohang Techno Park for the use of the ICP-MS (NexIon300x, Perkin Elmer) facility. The authors declare competing financial interests: {\textquoteleft}Composition for reducing arsenic accumulation{\textquoteright} is the subject of a Korea patent (10-2017-0060054 on May 15, 2017) applied by W-Y.S, Y.L and F.D. Publisher Copyright: {\textcopyright} 2018 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.",
year = "2018",
month = oct,
doi = "10.1111/pbi.12905",
language = "English",
volume = "16",
pages = "1691--1699",
journal = "Plant Biotechnology Journal",
issn = "1467-7644",
publisher = "Wiley-Blackwell",
number = "10",
}
