Enhanced Release of Chitosanase from Streptomyces griseus through Direct Interaction of Liposome with Cell Membrane under Heat Stress

Kien Xuan Ngo; Hiroshi Umakoshi; Toshinori Shimanouchi; Huong Thi Bui; Ryoichi Kuboi

doi:10.1263/jbb.106.602

Enhanced Release of Chitosanase from Streptomyces griseus through Direct Interaction of Liposome with Cell Membrane under Heat Stress

Kien Xuan Ngo, Hiroshi Umakoshi, Toshinori Shimanouchi, Huong Thi Bui, Ryoichi Kuboi

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

A direct interaction of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) liposomes with membrane of Streptomyces griseus cell under the heat stress at 41°C increased the chitosanase production and release to 2.2 times higher than that at 37°C without the POPC liposomes. Amount of chitosanase released across the lipid mimicking cell membrane (LMCM) liposome under a heat at 41°C in the presence of POPC liposomes was 17% of initially-entrapped chitosanase while it was only 1% in the absence of POPC liposomes, even under a heat stress at 41°C, clearly showing the importance of the direct interaction between membrane and membrane.

Original language	English
Pages (from-to)	602-605
Number of pages	4
Journal	Journal of Bioscience and Bioengineering
Volume	106
Issue number	6
DOIs	https://doi.org/10.1263/jbb.106.602
Publication status	Published - Dec 2008
Externally published	Yes

Keywords

Streptomyces griseus
chitosanase
heat stress
liposomes
membrane interaction

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

Access to Document

10.1263/jbb.106.602

Cite this

@article{3f27f639e8f64ea18eba959308417841,

title = "Enhanced Release of Chitosanase from Streptomyces griseus through Direct Interaction of Liposome with Cell Membrane under Heat Stress",

abstract = "A direct interaction of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) liposomes with membrane of Streptomyces griseus cell under the heat stress at 41°C increased the chitosanase production and release to 2.2 times higher than that at 37°C without the POPC liposomes. Amount of chitosanase released across the lipid mimicking cell membrane (LMCM) liposome under a heat at 41°C in the presence of POPC liposomes was 17% of initially-entrapped chitosanase while it was only 1% in the absence of POPC liposomes, even under a heat stress at 41°C, clearly showing the importance of the direct interaction between membrane and membrane.",

keywords = "Streptomyces griseus, chitosanase, heat stress, liposomes, membrane interaction",

author = "Ngo, {Kien Xuan} and Hiroshi Umakoshi and Toshinori Shimanouchi and Bui, {Huong Thi} and Ryoichi Kuboi",

note = "Funding Information: The fundamental concept of this study was supported by the Research Group of “Membrane Stress Biotechnology”. It was partially supported by a Grant-in-Aid for Scientific Research (nos. 15206089, 16686046, 16760635, and 17656268) from the Ministry of Education, Culture, Sports, Science and Technology of Japan, a grant from the Global COE program Bio-Environment Chemistry of Japan Society for the Promotion of Science (JSPS). The authors are grateful to the Research Center for Solar Energy Chemistry of Osaka University and the Gas Hydrate Analyzing System of Osaka University. One of the authors (K. X. Ngo) also deeply acknowledges the financial support of JSPS.",

year = "2008",

month = dec,

doi = "10.1263/jbb.106.602",

language = "English",

volume = "106",

pages = "602--605",

journal = "Journal of Bioscience and Bioengineering",

issn = "1389-1723",

publisher = "The Society for Biotechnology, Japan",

number = "6",

}

TY - JOUR

T1 - Enhanced Release of Chitosanase from Streptomyces griseus through Direct Interaction of Liposome with Cell Membrane under Heat Stress

AU - Ngo, Kien Xuan

AU - Umakoshi, Hiroshi

AU - Shimanouchi, Toshinori

AU - Bui, Huong Thi

AU - Kuboi, Ryoichi

N1 - Funding Information: The fundamental concept of this study was supported by the Research Group of “Membrane Stress Biotechnology”. It was partially supported by a Grant-in-Aid for Scientific Research (nos. 15206089, 16686046, 16760635, and 17656268) from the Ministry of Education, Culture, Sports, Science and Technology of Japan, a grant from the Global COE program Bio-Environment Chemistry of Japan Society for the Promotion of Science (JSPS). The authors are grateful to the Research Center for Solar Energy Chemistry of Osaka University and the Gas Hydrate Analyzing System of Osaka University. One of the authors (K. X. Ngo) also deeply acknowledges the financial support of JSPS.

PY - 2008/12

Y1 - 2008/12

N2 - A direct interaction of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) liposomes with membrane of Streptomyces griseus cell under the heat stress at 41°C increased the chitosanase production and release to 2.2 times higher than that at 37°C without the POPC liposomes. Amount of chitosanase released across the lipid mimicking cell membrane (LMCM) liposome under a heat at 41°C in the presence of POPC liposomes was 17% of initially-entrapped chitosanase while it was only 1% in the absence of POPC liposomes, even under a heat stress at 41°C, clearly showing the importance of the direct interaction between membrane and membrane.

AB - A direct interaction of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) liposomes with membrane of Streptomyces griseus cell under the heat stress at 41°C increased the chitosanase production and release to 2.2 times higher than that at 37°C without the POPC liposomes. Amount of chitosanase released across the lipid mimicking cell membrane (LMCM) liposome under a heat at 41°C in the presence of POPC liposomes was 17% of initially-entrapped chitosanase while it was only 1% in the absence of POPC liposomes, even under a heat stress at 41°C, clearly showing the importance of the direct interaction between membrane and membrane.

KW - Streptomyces griseus

KW - chitosanase

KW - heat stress

KW - liposomes

KW - membrane interaction

UR - http://www.scopus.com/inward/record.url?scp=58149105415&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=58149105415&partnerID=8YFLogxK

U2 - 10.1263/jbb.106.602

DO - 10.1263/jbb.106.602

M3 - Article

C2 - 19134559

AN - SCOPUS:58149105415

SN - 1389-1723

VL - 106

SP - 602

EP - 605

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

IS - 6

ER -

Enhanced Release of Chitosanase from Streptomyces griseus through Direct Interaction of Liposome with Cell Membrane under Heat Stress

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this