Enhancement of activity of sol-gel immobilized lipase in organic media by pretreatment with substrate analogues

Shin Ya Furukawa; Tsutomu Ono; Hiroyuki Ijima; Koei Kawakami

doi:10.1016/S1381-1177(01)00007-8

Enhancement of activity of sol-gel immobilized lipase in organic media by pretreatment with substrate analogues

Shin Ya Furukawa, Tsutomu Ono, Hiroyuki Ijima, Koei Kawakami

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

By performing pretreatment with substrate analogues, an activation of lipase immobilized into organic-inorganic hybrid silicate was attempted in esterification of menthol with butyric acid in organic media. With decreasing water content of immobilized lipase preparations, the activity of immobilized lipases by (-)-menthol pretreatment was enhanced, and the highest activity was obtained at water activity, a_w=0.11. However, the pretreatment of immobilized lipases with (-)-menthol could not improve the enantioselectivity. In addition, the activity of pretreated immobilized lipases increased with increasing the chain length of alcohol as a substrate analogue. Consequently, it was found that hydrophobic and bulky alcohol was an effective substrate analogue to activate immobilized lipases.

Original language	English
Pages (from-to)	65-70
Number of pages	6
Journal	Journal of Molecular Catalysis - B Enzymatic
Volume	15
Issue number	1-3
DOIs	https://doi.org/10.1016/S1381-1177(01)00007-8
Publication status	Published - Sept 28 2001
Externally published	Yes

Keywords

Esterification
Lipase
Organic-inorganic hybrid silicate
Sol-gel method
Substrate analogue

ASJC Scopus subject areas

Catalysis
Bioengineering
Biochemistry
Process Chemistry and Technology

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10.1016/S1381-1177(01)00007-8

Cite this

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abstract = "By performing pretreatment with substrate analogues, an activation of lipase immobilized into organic-inorganic hybrid silicate was attempted in esterification of menthol with butyric acid in organic media. With decreasing water content of immobilized lipase preparations, the activity of immobilized lipases by (-)-menthol pretreatment was enhanced, and the highest activity was obtained at water activity, aw=0.11. However, the pretreatment of immobilized lipases with (-)-menthol could not improve the enantioselectivity. In addition, the activity of pretreated immobilized lipases increased with increasing the chain length of alcohol as a substrate analogue. Consequently, it was found that hydrophobic and bulky alcohol was an effective substrate analogue to activate immobilized lipases.",

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T1 - Enhancement of activity of sol-gel immobilized lipase in organic media by pretreatment with substrate analogues

AU - Furukawa, Shin Ya

AU - Ono, Tsutomu

AU - Ijima, Hiroyuki

AU - Kawakami, Koei

PY - 2001/9/28

Y1 - 2001/9/28

N2 - By performing pretreatment with substrate analogues, an activation of lipase immobilized into organic-inorganic hybrid silicate was attempted in esterification of menthol with butyric acid in organic media. With decreasing water content of immobilized lipase preparations, the activity of immobilized lipases by (-)-menthol pretreatment was enhanced, and the highest activity was obtained at water activity, aw=0.11. However, the pretreatment of immobilized lipases with (-)-menthol could not improve the enantioselectivity. In addition, the activity of pretreated immobilized lipases increased with increasing the chain length of alcohol as a substrate analogue. Consequently, it was found that hydrophobic and bulky alcohol was an effective substrate analogue to activate immobilized lipases.

AB - By performing pretreatment with substrate analogues, an activation of lipase immobilized into organic-inorganic hybrid silicate was attempted in esterification of menthol with butyric acid in organic media. With decreasing water content of immobilized lipase preparations, the activity of immobilized lipases by (-)-menthol pretreatment was enhanced, and the highest activity was obtained at water activity, aw=0.11. However, the pretreatment of immobilized lipases with (-)-menthol could not improve the enantioselectivity. In addition, the activity of pretreated immobilized lipases increased with increasing the chain length of alcohol as a substrate analogue. Consequently, it was found that hydrophobic and bulky alcohol was an effective substrate analogue to activate immobilized lipases.

KW - Esterification

KW - Lipase

KW - Organic-inorganic hybrid silicate

KW - Sol-gel method

KW - Substrate analogue

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JF - Journal of Molecular Catalysis - B Enzymatic

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Enhancement of activity of sol-gel immobilized lipase in organic media by pretreatment with substrate analogues

Abstract

Keywords

ASJC Scopus subject areas

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