TY - JOUR
T1 - Enhancement of intestinal epithelial barrier function by Weissella confusa F213 and Lactobacillus rhamnosus FBB81 probiotic candidates in an in vitro model of hydrogen peroxide-induced inflammatory bowel disease
AU - Fatmawati, Ni Nengah Dwi
AU - Gotoh, Kazuyoshi
AU - Mayura, I. Putu Bayu
AU - Nocianitri, Komang Ayu
AU - Suwardana, Gede Ngurah Rsi
AU - Komalasari, Ni Luh Gede Yoni
AU - Ramona, Yan
AU - Sakaguchi, Masakiyo
AU - Matsushita, Osamu
AU - Sujaya, I. Nengah
N1 - Funding Information:
We would like to thank Professor Yukako Fujinaga (Department of Bacteriology, Graduate School of Medical Sciences, Kanazawa University, Japan) for providing Caco-2 cells. We also thank Nahoko Tomonobu (Department of Cell Biology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University) for the technical assistance, Gusti Putu Adi Wira Kusuma and I Gusti Putu Bhuana Aristya Putra who helped in maintaining the bacterial stocks and Heni Ruswita for the administration assistance.
Funding Information:
This study was supported by Udayana University through International Research Collaboration Grant Scheme with Grant No. B/20-68/UN14.4.A/PT.01.05/2020. Acknowledgements
Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12/1
Y1 - 2020/12/1
N2 - Objective: Weissella confusa F213 (WCF213) and Lactobacillus rhamnosus FBB81 (LrFBB81) are two probiotic candidates isolated from humans in our previous study. Their functional activity on the mucosal barrier has not yet been adequately investigated. Therefore, the objective of this study was to investigate the effect of these strains on maintaining mucosal integrity in vitro. Caco-2 cell monolayers were pretreated with WCF213 and LrFBB81 before being exposed to hydrogen peroxide. The integrity of mucosal cells was evaluated by measuring the transepithelial resistance (TER), flux of FITC-labelled dextran, and ZO-1 protein distribution with the help of an immunofluorescence method. Results: WCF213 was found to significantly maintain the TER better than the control hydrogen peroxide-treated cells (p < 0.001), followed by the strain combination, and LrFBB81 alone (p < 0.05). The permeability of mucosa was also successfully maintained by the WCF213 strain. This was illustrated by the significant reduction in the flux of FITC-labelled dextran (p < 0.05), which was larger than that exhibited by the other groups. The ZO-1 distribution of strain-treated cells showed less disruption than hydrogen peroxide-treated cells, consistent with the TER and FITC experimental results. These findings indicate that WCF213 and LrFBB81 plays important roles in the maintenance of mucosal integrity in a strain-dependent manner.
AB - Objective: Weissella confusa F213 (WCF213) and Lactobacillus rhamnosus FBB81 (LrFBB81) are two probiotic candidates isolated from humans in our previous study. Their functional activity on the mucosal barrier has not yet been adequately investigated. Therefore, the objective of this study was to investigate the effect of these strains on maintaining mucosal integrity in vitro. Caco-2 cell monolayers were pretreated with WCF213 and LrFBB81 before being exposed to hydrogen peroxide. The integrity of mucosal cells was evaluated by measuring the transepithelial resistance (TER), flux of FITC-labelled dextran, and ZO-1 protein distribution with the help of an immunofluorescence method. Results: WCF213 was found to significantly maintain the TER better than the control hydrogen peroxide-treated cells (p < 0.001), followed by the strain combination, and LrFBB81 alone (p < 0.05). The permeability of mucosa was also successfully maintained by the WCF213 strain. This was illustrated by the significant reduction in the flux of FITC-labelled dextran (p < 0.05), which was larger than that exhibited by the other groups. The ZO-1 distribution of strain-treated cells showed less disruption than hydrogen peroxide-treated cells, consistent with the TER and FITC experimental results. These findings indicate that WCF213 and LrFBB81 plays important roles in the maintenance of mucosal integrity in a strain-dependent manner.
KW - Inflammatory bowel diseases
KW - Lactobacillus rhamnosus FBB81
KW - Probiotics
KW - Trans epithelial resistance
KW - Weissella confusa F213
KW - ZO-1 protein
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U2 - 10.1186/s13104-020-05338-1
DO - 10.1186/s13104-020-05338-1
M3 - Article
C2 - 33081842
AN - SCOPUS:85092902968
SN - 1756-0500
VL - 13
JO - BMC Research Notes
JF - BMC Research Notes
IS - 1
M1 - 489
ER -