TY - JOUR
T1 - Enhancement of vascular permeability due to histamine-releasing effect of Vibrio vulnificus protease in rat skin
AU - Miyoshi, Shin ichi
AU - Sugiyama, Katsumi
AU - Suzuki, Yukio
AU - Furuta, Hiroaki
AU - Miyoshi, Noriko
AU - Shinoda, Sumio
PY - 1987/1
Y1 - 1987/1
N2 - Vibrio vulnificus protease, which had been purified from the culture supernatant of a strain isolated from a septicemic human patient, enhanced hypodermic vascular permeability and formed hemorrhagic spot caused by tissue destruction in a dose-dependent manner within a range of 0.3-10.0 μg when injected intradermally (i.d.) into rat dorsal skin. Enhancement of permeability was observed within 2 min, and the permeability-enhancing reaction terminated at 10 min after the injection. This reaction was blocked by simultaneous injection of an antihistamine, diphenhydramine, suggesting that the permeability-enhancing reaction of V. vulnificus protease was due to histamine release in vivo. Hemorrhage caused by V. vulnificus protease was also rapidly observed, but this reaction was not inhibited by the antihistamine.
AB - Vibrio vulnificus protease, which had been purified from the culture supernatant of a strain isolated from a septicemic human patient, enhanced hypodermic vascular permeability and formed hemorrhagic spot caused by tissue destruction in a dose-dependent manner within a range of 0.3-10.0 μg when injected intradermally (i.d.) into rat dorsal skin. Enhancement of permeability was observed within 2 min, and the permeability-enhancing reaction terminated at 10 min after the injection. This reaction was blocked by simultaneous injection of an antihistamine, diphenhydramine, suggesting that the permeability-enhancing reaction of V. vulnificus protease was due to histamine release in vivo. Hemorrhage caused by V. vulnificus protease was also rapidly observed, but this reaction was not inhibited by the antihistamine.
KW - (Vascular permeability enhancement)
KW - Histamine
KW - Protease
KW - Vibrio vulnificus
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U2 - 10.1111/j.1574-6968.1987.tb01989.x
DO - 10.1111/j.1574-6968.1987.tb01989.x
M3 - Article
AN - SCOPUS:0023109713
SN - 0378-1097
VL - 40
SP - 95
EP - 98
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 1
ER -