TY - JOUR
T1 - Enhancing effect of saccharides on the mutagenicity of 2-chloro-4-methylthiobutanoic acid
AU - Kimura, Sachiko
AU - Arimoto-Kobayashi, Sakae
AU - Hayatsu, Hikoya
N1 - Funding Information:
This work was supported by the Grant-in-Aid for Cancer Research (8-5) from the Ministry of Health and Welfare. Professor Masayoshi Eto of the Himeji Institute of Technology is thanked for his kind encouragement to S.K. throughout this work. We are grateful to Dr. Yohoko Shono of the same institute for her valuable discussions.
PY - 2000/7/20
Y1 - 2000/7/20
N2 - The mutagenicity of 2-chloro-4-methylthiobutanoic acid (CMBA), a nitrite-treated Sanma fish mutagen, in Salmonella typhimurium TA100 was enhanced by addition of D-glucose during the CMBA-treatment. Several other monosaccharides also enhanced the mutagenicity of CMBA, and the order of the enhancing potency was found to be D-mannose, D-glucose>D-fructose, D-ribose, D-galactose. A disaccharide, maltose, showed only little enhancement. No enhancement was found with L-glucose. We investigated whether saccharides affect uptake of [methyl-14C]CMBA into S. typhimurium TA100. Saccharides which enhanced CMBA-induced mutagenesis increased the uptake. L-Glucose did not enhance the uptake. There was good correlation between the enhanced mutagenesis and increased radioactivity in Salmonella, suggesting that the enhancing effect of monosaccharide on the CMBA-induced mutagenesis results from the enhanced uptake of the mutagen into bacteria. Copyright (C) 2000 Elsevier Science B.V.
AB - The mutagenicity of 2-chloro-4-methylthiobutanoic acid (CMBA), a nitrite-treated Sanma fish mutagen, in Salmonella typhimurium TA100 was enhanced by addition of D-glucose during the CMBA-treatment. Several other monosaccharides also enhanced the mutagenicity of CMBA, and the order of the enhancing potency was found to be D-mannose, D-glucose>D-fructose, D-ribose, D-galactose. A disaccharide, maltose, showed only little enhancement. No enhancement was found with L-glucose. We investigated whether saccharides affect uptake of [methyl-14C]CMBA into S. typhimurium TA100. Saccharides which enhanced CMBA-induced mutagenesis increased the uptake. L-Glucose did not enhance the uptake. There was good correlation between the enhanced mutagenesis and increased radioactivity in Salmonella, suggesting that the enhancing effect of monosaccharide on the CMBA-induced mutagenesis results from the enhanced uptake of the mutagen into bacteria. Copyright (C) 2000 Elsevier Science B.V.
KW - 2-Chloro-4-methylthiobutanoic acid
KW - D-Glucose
KW - Mutagenesis enhancement
KW - Saccharide
KW - Sanma-fish mutagen
KW - [Methyl-C]2-chloro-4-methylthiobutanoic acid
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U2 - 10.1016/S0027-5107(00)00061-0
DO - 10.1016/S0027-5107(00)00061-0
M3 - Article
C2 - 10894898
AN - SCOPUS:0034691694
SN - 0027-5107
VL - 452
SP - 139
EP - 144
JO - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
JF - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
IS - 1
ER -