Enhancing effect of saccharides on the mutagenicity of 2-chloro-4-methylthiobutanoic acid

Sachiko Kimura, Sakae Arimoto-Kobayashi, Hikoya Hayatsu

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


The mutagenicity of 2-chloro-4-methylthiobutanoic acid (CMBA), a nitrite-treated Sanma fish mutagen, in Salmonella typhimurium TA100 was enhanced by addition of D-glucose during the CMBA-treatment. Several other monosaccharides also enhanced the mutagenicity of CMBA, and the order of the enhancing potency was found to be D-mannose, D-glucose>D-fructose, D-ribose, D-galactose. A disaccharide, maltose, showed only little enhancement. No enhancement was found with L-glucose. We investigated whether saccharides affect uptake of [methyl-14C]CMBA into S. typhimurium TA100. Saccharides which enhanced CMBA-induced mutagenesis increased the uptake. L-Glucose did not enhance the uptake. There was good correlation between the enhanced mutagenesis and increased radioactivity in Salmonella, suggesting that the enhancing effect of monosaccharide on the CMBA-induced mutagenesis results from the enhanced uptake of the mutagen into bacteria. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)139-144
Number of pages6
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Issue number1
Publication statusPublished - Jul 20 2000


  • 2-Chloro-4-methylthiobutanoic acid
  • D-Glucose
  • Mutagenesis enhancement
  • Saccharide
  • Sanma-fish mutagen
  • [Methyl-C]2-chloro-4-methylthiobutanoic acid

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Health, Toxicology and Mutagenesis


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