Enzyme-linked immunosorbent assay for screening of canine brucellosis using recombinant Cu-Zn superoxide dismutase

Ganbat Tsogtbaatar; Masato Tachibana; Kenta Watanabe; Suk Kim; Hiroshi Suzuki; Masahisa Watarai

doi:10.1292/jvms.70.1387

Enzyme-linked immunosorbent assay for screening of canine brucellosis using recombinant Cu-Zn superoxide dismutase

Ganbat Tsogtbaatar, Masato Tachibana, Kenta Watanabe, Suk Kim, Hiroshi Suzuki, Masahisa Watarai

Dental School

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Brucella canis, a facultative intracellular pathogen, is the causative agent of canine brucellosis. The diagnosis of canine brucellosis is based on bacteriological examination and serological methods including agglutination and gel diffusion tests. In this study, recombinant B. canis Cu-Zn superoxide dismutase (SOD) was used as an antigen for the enzyme-linked immunosorbent assay (ELISA). The recombinant SOD showed a specific reaction with serum infected with B. canis in Western blotting and ELISA. These results suggest that ELISA using recombinant SOD is useful in screening for canine brucellosis.

Original language	English
Pages (from-to)	1387-1389
Number of pages	3
Journal	Journal of Veterinary Medical Science
Volume	70
Issue number	12
DOIs	https://doi.org/10.1292/jvms.70.1387
Publication status	Published - Dec 2008

Keywords

Brucellosis
Cu-Zn superoxide dismutase
ELISA

ASJC Scopus subject areas

veterinary(all)

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10.1292/jvms.70.1387

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abstract = "Brucella canis, a facultative intracellular pathogen, is the causative agent of canine brucellosis. The diagnosis of canine brucellosis is based on bacteriological examination and serological methods including agglutination and gel diffusion tests. In this study, recombinant B. canis Cu-Zn superoxide dismutase (SOD) was used as an antigen for the enzyme-linked immunosorbent assay (ELISA). The recombinant SOD showed a specific reaction with serum infected with B. canis in Western blotting and ELISA. These results suggest that ELISA using recombinant SOD is useful in screening for canine brucellosis.",

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AU - Tsogtbaatar, Ganbat

AU - Tachibana, Masato

AU - Watanabe, Kenta

AU - Kim, Suk

AU - Suzuki, Hiroshi

AU - Watarai, Masahisa

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AB - Brucella canis, a facultative intracellular pathogen, is the causative agent of canine brucellosis. The diagnosis of canine brucellosis is based on bacteriological examination and serological methods including agglutination and gel diffusion tests. In this study, recombinant B. canis Cu-Zn superoxide dismutase (SOD) was used as an antigen for the enzyme-linked immunosorbent assay (ELISA). The recombinant SOD showed a specific reaction with serum infected with B. canis in Western blotting and ELISA. These results suggest that ELISA using recombinant SOD is useful in screening for canine brucellosis.

KW - Brucellosis

KW - Cu-Zn superoxide dismutase

KW - ELISA

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Enzyme-linked immunosorbent assay for screening of canine brucellosis using recombinant Cu-Zn superoxide dismutase

Abstract

Keywords

ASJC Scopus subject areas

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