TY - JOUR
T1 - Epigenomic modification in rice controls meiotic recombination and segregation distortion
AU - Habu, Yoshiki
AU - Ando, Tsuyu
AU - Ito, Sachie
AU - Nagaki, Kiyotaka
AU - Kishimoto, Naoki
AU - Taguchi-Shiobara, Fumio
AU - Numa, Hisataka
AU - Yamaguchi, Katsushi
AU - Shigenobu, Shuji
AU - Murata, Minoru
AU - Meshi, Tetsuo
AU - Yano, Masahiro
N1 - Funding Information:
We thank K.-I. Nonomua for the RCS2 centromeric probe; T. Kanno and H. Rothnie for their comments on this study; K. Hioki, H. Onodera and A. Tagiri for technical assistance. This work was supported by grants of Promotion of Research Activity and NIAS Strategic Research Fund from National Institute of Agrobiological Sciences, NIBB Cooperative Research Program (Next-generation DNA Sequencing Initiative: 11-703), the Ministry of Agriculture, Forestry and Fisheries (PGE1004), and JST/CREST, Japan.
Publisher Copyright:
© 2015, Springer Science+Business Media Dordrecht.
PY - 2015/4/1
Y1 - 2015/4/1
N2 - The low frequency of meiotic recombination in chromosomal regions other than hotspots is a general obstacle to efficient breeding. A number of active genes are present in recombination-repressed centromeric regions in higher eukaryotes, suggesting that suppression of meiotic recombination prevents shuffling of genes within a centromeric region. In this study, by using an inter-subspecific cross of Oryza sativa L., we show that modification of inactive chromatin states by either genetic or chemical inhibition of chromatin modifying proteins induced changes in both the position of meiotic recombination and, unexpectedly, the pattern of segregation distortion of parental alleles. Antisense knockdown of rice homologues of DECREASE IN DNA METHYLATION1, which is required for the maintenance of heterochromatin in Arabidopsis thaliana, induced a recombination hotspot in a centromeric region accompanied by a steep increase in the proportion of heterozygotes. Our results describe a previously undocumented phenomenon in which artificial chromatin modification could be used to change the pattern of segregation distortion in rice and open up novel possibilities for efficient crop breeding.
AB - The low frequency of meiotic recombination in chromosomal regions other than hotspots is a general obstacle to efficient breeding. A number of active genes are present in recombination-repressed centromeric regions in higher eukaryotes, suggesting that suppression of meiotic recombination prevents shuffling of genes within a centromeric region. In this study, by using an inter-subspecific cross of Oryza sativa L., we show that modification of inactive chromatin states by either genetic or chemical inhibition of chromatin modifying proteins induced changes in both the position of meiotic recombination and, unexpectedly, the pattern of segregation distortion of parental alleles. Antisense knockdown of rice homologues of DECREASE IN DNA METHYLATION1, which is required for the maintenance of heterochromatin in Arabidopsis thaliana, induced a recombination hotspot in a centromeric region accompanied by a steep increase in the proportion of heterozygotes. Our results describe a previously undocumented phenomenon in which artificial chromatin modification could be used to change the pattern of segregation distortion in rice and open up novel possibilities for efficient crop breeding.
KW - DECREASE IN DNA METHYLATION1
KW - Meiotic recombination
KW - Rice
KW - Segregation distortion
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U2 - 10.1007/s11032-015-0299-0
DO - 10.1007/s11032-015-0299-0
M3 - Article
AN - SCOPUS:84925857721
SN - 1380-3743
VL - 35
JO - Molecular Breeding
JF - Molecular Breeding
IS - 4
ER -