TY - JOUR
T1 - Epstein-Barr virus-associated primary central nervous system lymphomas in immunocompetent elderly patients
T2 - Analysis for latent membrane protein-1 oncogene deletion and EBNA-2 strain typing
AU - Sugita, Yasuo
AU - Terasaki, Mizuhiko
AU - Niino, Daisuke
AU - Ohshima, Koichi
AU - Fumiko, Arakawa
AU - Shigemori, Minoru
AU - Sato, Yasuharu
AU - Asano, Naoko
N1 - Funding Information:
Acknowledgments This work was supported by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology, Japan (2008–2010). We would like to thank Dr Daisuke Mori (Saga Prefectural Hospital) for providing valuable samples (case 1).
PY - 2010/11
Y1 - 2010/11
N2 - Epstein-Barr virus (EBV) has been implicated in the pathogenesis of primary central nervous system lymphomas (PCNLs) in immunocompetent hosts. To investigate the role of EBV in the pathogenesis of PCNLs in immunocompetent hosts, this study assessed six PCNL cases (elderly male immunocompetent patients; age ≥60 years) histologically and immunohistochemically, and an EBV genetic study was performed. Histologically, all cases were diagnosed as diffuse large B-cell lymphoma with extensive necrosis. In all six cases, PCNL cells showed immunohistochemical positivity for latent membrane protein 1 (LMP-1) and Epstein-Barr nuclear 2 (EBNA2). Lymphoma cells also showed positive signals for EBV-encoded small RNAs (EBERs) on in-situ hybridization. EBV subtyping-PCR analysis demonstrated that one case was EBNA 2B type and the other five cases were EBNA 2A type, and two cases were EBV wild-type and four cases showed 30-bp LMP-1 deletion by PCR analysis. It is therefore possible that LMP gene deletion or EBNA-2 strain type are important in the tumorigenesis of EBV-positive PCNLs. In addition, EBV-positive PCNLs in immunocompetent hosts may be related to immunological deterioration derived from the aging process.
AB - Epstein-Barr virus (EBV) has been implicated in the pathogenesis of primary central nervous system lymphomas (PCNLs) in immunocompetent hosts. To investigate the role of EBV in the pathogenesis of PCNLs in immunocompetent hosts, this study assessed six PCNL cases (elderly male immunocompetent patients; age ≥60 years) histologically and immunohistochemically, and an EBV genetic study was performed. Histologically, all cases were diagnosed as diffuse large B-cell lymphoma with extensive necrosis. In all six cases, PCNL cells showed immunohistochemical positivity for latent membrane protein 1 (LMP-1) and Epstein-Barr nuclear 2 (EBNA2). Lymphoma cells also showed positive signals for EBV-encoded small RNAs (EBERs) on in-situ hybridization. EBV subtyping-PCR analysis demonstrated that one case was EBNA 2B type and the other five cases were EBNA 2A type, and two cases were EBV wild-type and four cases showed 30-bp LMP-1 deletion by PCR analysis. It is therefore possible that LMP gene deletion or EBNA-2 strain type are important in the tumorigenesis of EBV-positive PCNLs. In addition, EBV-positive PCNLs in immunocompetent hosts may be related to immunological deterioration derived from the aging process.
KW - EBNA-2 strain typing
KW - Epstein-Barr virus
KW - Immunocompetent host
KW - Latent membrane protein-1 oncogene deletion
KW - Primary central nervous system lymphoma
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U2 - 10.1007/s11060-010-0191-z
DO - 10.1007/s11060-010-0191-z
M3 - Article
C2 - 20455004
AN - SCOPUS:79952183507
SN - 0167-594X
VL - 100
SP - 271
EP - 279
JO - Journal of neuro-oncology
JF - Journal of neuro-oncology
IS - 2
ER -
