Erratum: Autocrine regulation of ecdysone synthesis by β3-octopamine receptor in the prothoracic gland is essential for Drosophila metamorphosis(Proc. Natl. Acad. Sci. U.S.A. (2015) 112 (1452-1457) DOI: 10.1073/pnas.1414966112)

DEVELOPMENTAL BIOLOGY Correction for “Autocrine regulation of ecdysone synthesis by β3-octopamine receptor in the prothoracic gland is essential for Drosophila metamorphosis,” by Yuya Ohhara, Yuko Shimada-Niwa, Ryusuke Niwa, Yasunari Kayashima, Yoshiki Hayashi, Kazutaka Akagi, Hitoshi Ueda, Kimiko Yamakawa-Kobayashi, and Satoru Kobayashi, which was first published January 20, 2015; 10.1073/ pnas.1414966112 (Proc. Natl. Acad. Sci. U.S.A. 112, 1452-1457). The authors wish to note the following: “We used two RNAi lines expressing dsRNA against Tyrosine decarboxylase 2 (Tdc2): Tdc2 RNAi-1 (10687R-1) and Tdc2 RNAi-2 (BL#25871) obtained from the National Institute of Genetics (NIG, Japan) fly stock center and the Bloomington Drosophila Stock Center, respectively. We found that knockdown of Tdc2 by using Tdc2 RNAi-1 and Tdc2 RNAi-2 in the prothoracic gland (PG) both caused impairment in developmental transition from larval to pupal stage. However, the NIG fly stock center informed us that Tdc2 RNAi-1 line possesses an RNAi construct against Asparaginyl-tRNA synthetase (AsnRS) instead of Tdc2. It is uncertain how the expression of AsnRS RNAi results in Tdc2 knockdown. “To confirm that Tdc2 knockdown in PG causes a defect in developmental transition from larvae to pupae, we performed additional experiments using Tdc2 RNAi-2 and Tdc2 short hairpin RNA line (v330541), which has been newly obtained from the Vienna Drosophila RNAi Center (designated as Tdc2 RNAi-3 hereafter). In brief, expression of Tdc2 RNAi-2 construct caused Tdc2 down-regulation in the PG, whereas Tdc2 expression level was not reduced in the PG expressing Tdc2 RNAi-3. Accordingly, the timing of pupariation was delayed in Tdc2 RNAi-2 animals and ∼40% of them arrested at the larval stage. In contrast, Tdc2 RNAi-3 animals showed only a slight delay in larval-pupal transition. Furthermore, when Tdc2 RNAi-2 animals were raised at 28 °C to enhance transcription of the RNAi construct, all Tdc2 RNAi-2 animals were arrested at the larval stage. These results support our original conclusion that Tdc2 in the PG is required for larval to pupal transition. We can provide any additional information upon request.