Essential cys-pro-cys motif of caenorhabditis elegans copper transport atpase

Takao Yoshimizu; Hiroshi Omote; Tokumitsu Wakabayashi; Yoshihiro Sambongi; Masamitsu Futai

doi:10.1271/bbb.62.1258

Essential cys-pro-cys motif of caenorhabditis elegans copper transport atpase

Takao Yoshimizu, Hiroshi Omote, Tokumitsu Wakabayashi, Yoshihiro Sambongi, Masamitsu Futai

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

Caenorhabditis elegans putative copper ATPase (CUA-1) had been functionally expressed in a yeast Δccc2 mutant (copper ATPase gene disruptant). We found that CUA-1 with Cys-Pro-Cys to Cys-Pro-Ala mutation could not rescue the yeast Δccc2 mutant, suggesting that the carboxyl terminal cysteine residue in the conserved Cys-Pro-Cys motif is essential for copper transport.

Original language	English
Pages (from-to)	1258-1260
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	62
Issue number	6
DOIs	https://doi.org/10.1271/bbb.62.1258
Publication status	Published - Jan 1 1998
Externally published	Yes

Keywords

Caenorhabditis elegans
Copper ATPase
Cysteine residue
Menkes and Wilson diseases
Yeast CCC2 gene

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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abstract = "Caenorhabditis elegans putative copper ATPase (CUA-1) had been functionally expressed in a yeast Δccc2 mutant (copper ATPase gene disruptant). We found that CUA-1 with Cys-Pro-Cys to Cys-Pro-Ala mutation could not rescue the yeast Δccc2 mutant, suggesting that the carboxyl terminal cysteine residue in the conserved Cys-Pro-Cys motif is essential for copper transport.",

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AU - Yoshimizu, Takao

AU - Omote, Hiroshi

AU - Wakabayashi, Tokumitsu

AU - Sambongi, Yoshihiro

AU - Futai, Masamitsu

PY - 1998/1/1

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N2 - Caenorhabditis elegans putative copper ATPase (CUA-1) had been functionally expressed in a yeast Δccc2 mutant (copper ATPase gene disruptant). We found that CUA-1 with Cys-Pro-Cys to Cys-Pro-Ala mutation could not rescue the yeast Δccc2 mutant, suggesting that the carboxyl terminal cysteine residue in the conserved Cys-Pro-Cys motif is essential for copper transport.

AB - Caenorhabditis elegans putative copper ATPase (CUA-1) had been functionally expressed in a yeast Δccc2 mutant (copper ATPase gene disruptant). We found that CUA-1 with Cys-Pro-Cys to Cys-Pro-Ala mutation could not rescue the yeast Δccc2 mutant, suggesting that the carboxyl terminal cysteine residue in the conserved Cys-Pro-Cys motif is essential for copper transport.

KW - Caenorhabditis elegans

KW - Copper ATPase

KW - Cysteine residue

KW - Menkes and Wilson diseases

KW - Yeast CCC2 gene

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Essential cys-pro-cys motif of caenorhabditis elegans copper transport atpase

Abstract

Keywords

ASJC Scopus subject areas

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