Establishment of a human pluripotent stem cell-derived MKX-td Tomato reporter system

Yuki Fujisawa, Lu Ming, Daisuke Yamada, Tomoka Takao, Takeshi Takarada

Research output: Contribution to journalArticlepeer-review


Tendon regeneration is difficult because detailed knowledge about tendon progenitor cells (TPCs), which produce tenocytes to repair tendon tissue, has not been revealed. Mohawk homeobox (MKX) is a marker of TPCs or tenocytes, but a human pluripotent stem cell (hPSC)-based reporter system that visualizes MKX+ cells has not been developed. Here, we established an hPSC-derived MKX-tdTomato reporter cell line and tested the induction ratio of MKX-tdTomato+ cells using our stepwise/xeno-free differentiation protocol. MKX-tdTomato+ cells were generated with high efficiency and expressed tendon-specific markers, including MKX, SCX, TNMD, and COL1A1. Our MKX-tdTomato hPSC line would be a useful tool for studying the development or regeneration of tendon tissue.

Original languageEnglish
Article number515
JournalStem Cell Research and Therapy
Issue number1
Publication statusPublished - Dec 2022


  • Human pluripotent stem cells
  • Tendon
  • Tendon regeneration
  • Tenocytes

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Cell Biology


Dive into the research topics of 'Establishment of a human pluripotent stem cell-derived MKX-td Tomato reporter system'. Together they form a unique fingerprint.

Cite this