Establishment of a monoclonal antibody against cd81 that decreases the proliferation of rat glioma cells

Erika Fujimoto; Hiroki Mori; Motoharu Takehara; Miyuki Tanaka; Toshitaka Ohashi; Yoshifumi Ninomiya; Tohru Nakanishi

doi:10.2485/jhtb.23.131

Establishment of a monoclonal antibody against cd81 that decreases the proliferation of rat glioma cells

Erika Fujimoto, Hiroki Mori, Motoharu Takehara, Miyuki Tanaka, Toshitaka Ohashi, Yoshifumi Ninomiya, Tohru Nakanishi

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

CD81 belongs to a family of cell-surface proteins (tetraspanins) known to be up-regulated in rheumatoid arthritis (RA) synoviocytes. Recently, we showed that small interfering RNA targeting CD81 (CD81 siRNA) has a therapeutic effect on RA. We also showed that CD81 siRNA decreased the expression of TNF- in SW982 cells, suggesting that TNF- stimulates the expression of synoviolin via CD81. Here, we show that a monoclonal antibody (MAb) against CD81, which was raised by immunization with recombinant CD81, decreased the proliferation of rat C6 glioma cells, showing that this antibody is functionally active and probably inhibits cell proliferation through the inhibition of CD81 function on the cell surface.

Original language	English
Pages (from-to)	131-134
Number of pages	4
Journal	Journal of Hard Tissue Biology
Volume	23
Issue number	1
DOIs	https://doi.org/10.2485/jhtb.23.131
Publication status	Published - 2014

Keywords

C6 glioma
Cd81
Monoclonal antibody
Rheumatoid arthritis

ASJC Scopus subject areas

Medicine (miscellaneous)
Biochemistry
Biomaterials
Orthopedics and Sports Medicine
Dentistry(all)
Cell Biology

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10.2485/jhtb.23.131

Cite this

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abstract = "CD81 belongs to a family of cell-surface proteins (tetraspanins) known to be up-regulated in rheumatoid arthritis (RA) synoviocytes. Recently, we showed that small interfering RNA targeting CD81 (CD81 siRNA) has a therapeutic effect on RA. We also showed that CD81 siRNA decreased the expression of TNF- in SW982 cells, suggesting that TNF- stimulates the expression of synoviolin via CD81. Here, we show that a monoclonal antibody (MAb) against CD81, which was raised by immunization with recombinant CD81, decreased the proliferation of rat C6 glioma cells, showing that this antibody is functionally active and probably inhibits cell proliferation through the inhibition of CD81 function on the cell surface.",

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AU - Fujimoto, Erika

AU - Mori, Hiroki

AU - Takehara, Motoharu

AU - Tanaka, Miyuki

AU - Ohashi, Toshitaka

AU - Ninomiya, Yoshifumi

AU - Nakanishi, Tohru

PY - 2014

Y1 - 2014

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AB - CD81 belongs to a family of cell-surface proteins (tetraspanins) known to be up-regulated in rheumatoid arthritis (RA) synoviocytes. Recently, we showed that small interfering RNA targeting CD81 (CD81 siRNA) has a therapeutic effect on RA. We also showed that CD81 siRNA decreased the expression of TNF- in SW982 cells, suggesting that TNF- stimulates the expression of synoviolin via CD81. Here, we show that a monoclonal antibody (MAb) against CD81, which was raised by immunization with recombinant CD81, decreased the proliferation of rat C6 glioma cells, showing that this antibody is functionally active and probably inhibits cell proliferation through the inhibition of CD81 function on the cell surface.

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Establishment of a monoclonal antibody against cd81 that decreases the proliferation of rat glioma cells

Abstract

Keywords

ASJC Scopus subject areas

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